Human Myeloperoxidase/MPO Antibody

Catalog #: MAB31741 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB31741-100
MAB31741-SP

Human Myeloperoxidase/MPO ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Myeloperoxidase/MPO Antibody Summary

Species Reactivity

Human

Specificity

Detects human Myeloperoxidase/MPO in sandwich ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 358621

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived human Myeloperoxidase/MPO protein
Ala49-Ser745
Accession # P05164

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody.

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Myeloperoxidase DuoSet ELISA Kit (Catalog # DY3174) for convenient development of a sandwich ELISA or the Human Myeloperoxidase Quantikine ELISA Kit (Catalog # DMYE00B) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Myeloperoxidase/MPO ELISA Standard Curve. Recombinant Human Myeloperoxidase/MPO protein was serially diluted 2-fold and captured by Rat Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB31741) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myeloperoxidase/MPO

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Human and mouse MPO share 87% amino acid sequence identity.

Entrez Gene IDs

4353 (Human); 17523 (Mouse); 303413 (Rat)

Alternate Names

EC 1.11.1; EC 1.11.1.7; MPO; Myeloperoxidase