Human MS4A3 Antibody

Catalog #: MAB6247 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6247
MAB6247-SP

Detection of MS4A3 in Human PBMC by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MS4A3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MS4A3 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 489433

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human MS4A3
Accession # NP_006129

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MS4A3 in Human PBMC by Flow Cytometry. Human peripheral blood monocytes were stained with Human MS4A3 Monoclonal Antibody (Catalog # MAB6247, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0103B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MS4A3

MS4A3, also known as HTm4, is an intracytoplasmic membrane protein that is a member of the MS4A family of four-transmembrane proteins that also includes CD20 and Fc epsilon RI beta. It is a cell cycle regulator expressed in the perinuclear area of human hematopoietic cells. The C-terminal region of MS4A3 interacts with cdc-associated phosphatase (KAP), enhancing its activity on cyclin-dependent kinase 2 (cdk2) and facilitating G(0)/G(1) arrest. The combined non-membrane segments of the 214 amino acid (aa), 25 kDa human MS4A3 show 53% and 52% aa identity with corresponding regions of mouse and rat MS4A3, respectively.

Long Name

Membrane-spanning 4-domains, Subfamily A, Member 3 [Hematopoietic Cell-specific]

Entrez Gene IDs

932 (Human); 170813 (Mouse); 293753 (Rat)

Alternate Names

CD20 antigen homolog; CD20 antigen-like protein; CD20L; CD20LIgE receptor beta subunit; hematopoietic cell 4 transmembrane protein; Hematopoietic-specific transmembrane protein 4; HTM4; HTM4IgE receptor beta chain; membrane-spanning 4-domains subfamily A member 3; membrane-spanning 4-domains, subfamily A, member 3 (hematopoieticcell-specific); MS4A3