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Human/Mouse UCP1 Antibody

Catalog #: MAB6158
50 Citations 2 Reviews Datasheet / COA / SDS
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MAB6158
MAB6158-SP
Detection of Human UCP1 by Western Blot.
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Product Details
Citations (50)
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Reviews (2)
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Human/Mouse UCP1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse UCP1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 536435
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human UCP1
Met1-Thr307
Accession # P25874
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human UCP1 antibody by Western Blot. View Larger

Detection of Human UCP1 by Western Blot. Western blot shows recombinant human UCP1, recombinant human UCP2, recombinant human UCP3, and recombinant human UCP4 (5 ng/lane). PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western Blot Detection of Mouse UCP1 antibody by Western Blot. View Larger

Detection of Mouse UCP1 by Western Blot. Western blot shows lysates of mouse brown adipose tissue and mouse adipose tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UCP1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry UCP1 antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

UCP1 in Human Mesenchymal Stem Cells. UCP1 was detected in immersion fixed human mesenchymal stem cells undifferentiated (lower panel) or differentiated into adipocytes (upper panel) using Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of UCP1 antibody in 3T3-L1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of UCP1 in 3T3-L1 Mouse Cell Line by Flow Cytometry. 3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Simple Western Detection of Mouse UCP1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse UCP1 by Simple WesternTM. Simple Western lane view shows lysates of mouse adipose tissue and mouse brown adipose tissue, loaded at 0.5 mg/mL. A specific band was detected for UCP1 at approximately 37 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry Detection of Mouse UCP1 by Immunohistochemistry View Larger

Detection of Mouse UCP1 by Immunohistochemistry IRX3/Irx3 expression is induced in browning adipose tissues.(A) Relative mRNA levels of Ucp1 and Irx3 in IWAT from mice under cold stress (4 °C) for one week (n = 6) and at 25 °C (n = 8). (B–C) Relative mRNA levels of Ucp1 and Irx3 in IWAT (B) and BAT (C) from mice subjected to PBS or CL-316,243 for 10 days. For A–C, gene expression was normalized to 36b4. (D–E) Protein levels of Ucp1 and Irx3 (left) and the quantification value of Irx3 relative to Hsp90 (right) in IWAT (D) and BAT (E) in the mice subjected to PBS or CL-316,243 treatment. (F) Representative images of immunohistochemical staining in IWAT from mice subjected to PBS or CL-316,243 treatment (scale bar, 50 μm for the upper and middle panels, and 100 μm for the bottom panel). (G) PET-CT, gross image, HE staining (scale bar, 20 μm), and electron microscopy (scale bar, 5 μm) of the browning WAT from pheochromocytoma patients. (H) Representative images of immunohistochemical staining in oWAT from pheochromocytoma patients (scale bar, 100 μm for the upper panel, and 50 μm for the bottom panel). Data were presented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. The results are representative of at least three independent experiments. IWAT, inguinal white adipose tissue. BAT, brown adipose tissue. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28988979), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse UCP1 by Immunohistochemistry View Larger

Detection of Mouse UCP1 by Immunohistochemistry IRX3/Irx3 expression is induced in browning adipose tissues.(A) Relative mRNA levels of Ucp1 and Irx3 in IWAT from mice under cold stress (4 °C) for one week (n = 6) and at 25 °C (n = 8). (B–C) Relative mRNA levels of Ucp1 and Irx3 in IWAT (B) and BAT (C) from mice subjected to PBS or CL-316,243 for 10 days. For A–C, gene expression was normalized to 36b4. (D–E) Protein levels of Ucp1 and Irx3 (left) and the quantification value of Irx3 relative to Hsp90 (right) in IWAT (D) and BAT (E) in the mice subjected to PBS or CL-316,243 treatment. (F) Representative images of immunohistochemical staining in IWAT from mice subjected to PBS or CL-316,243 treatment (scale bar, 50 μm for the upper and middle panels, and 100 μm for the bottom panel). (G) PET-CT, gross image, HE staining (scale bar, 20 μm), and electron microscopy (scale bar, 5 μm) of the browning WAT from pheochromocytoma patients. (H) Representative images of immunohistochemical staining in oWAT from pheochromocytoma patients (scale bar, 100 μm for the upper panel, and 50 μm for the bottom panel). Data were presented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. The results are representative of at least three independent experiments. IWAT, inguinal white adipose tissue. BAT, brown adipose tissue. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28988979), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human UCP1 by Western Blot View Larger

Detection of Human UCP1 by Western Blot BMP7 upregulated browning markers in both SC and DN derived differentiated adipocytes. SC and DN preadipocytes were differentiated and treated as in Figure 1. (A) Browning content as estimated by BATLAS Webtool (n = 9); (B) texture sum variance as quantified by laser-scanning cytometry (n = 5); quantification of UCP1 gene expression by (C) RNA-sequencing (n = 9) and (D) RT-qPCR normalized to GAPDH (n = 5). (E) UCP1 protein expression normalized to beta -actin (n = 5); (F) representative UCP1 immunostaining images visualized by confocal microscopy. Nucleus was stained using propidium iodide (PI), BF represents bright field image. Scale bars represent 10 μm. (G) Quantification of UCP1 immunostaining intensity assisted by laser-scanning cytometry (n = 4000 cells of 4 donors). Data expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Statistics: Paired t-test (A,B,G), GLM (C), and one-way ANOVA with Tukey’s post-test (D,E). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34832860), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UCP1

Mitochondrial brown fat uncoupling protein 1 (UCP1; also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human and mouse UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Human and mouse UCP1 share 79% aa sequence identity.

Long Name
Uncoupling Protein 1
Entrez Gene IDs
7350 (Human); 22227 (Mouse); 24860 (Rat)
Alternate Names
SLC25A7; SLC25A7mitochondrial brown fat uncoupling protein 1; Solute carrier family 25 member 7; Thermogenin; UCP 1; UCP; UCP1; uncoupling protein 1 (mitochondrial, proton carrier)

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Citations for Human/Mouse UCP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

50 Citations: Showing 1 - 10
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  1. Reprogrammed Functional Brown Adipocytes Ameliorate Insulin Resistance and Dyslipidemia in Diet-Induced Obesity and Type 2 Diabetes.
    Authors: Kishida T, Ejima A, Yamamoto K et al.
    Stem Cell Reports
  2. BMP7 increases UCP1-dependent and independent thermogenesis with a unique gene expression program in human neck area derived adipocytes
    Authors: Shaw A, TOth B, Arianti R Et al.
    Pharmaceuticals
  3. Rab27a Regulates Human Perivascular Adipose Progenitor Cell Differentiation
    Authors: Joshua M. Boucher, Michael Robich, S. Spencer Scott, Xuehui Yang, Larisa Ryzhova, Jacqueline E. Turner et al.
    Cardiovascular Drugs and Therapy
  4. Preventing White Adipocyte Browning during Differentiation In Vitro: The Effect of Differentiation Protocols on Metabolic and Mitochondrial Phenotypes
    Authors: E Herbers, M Patrikoski, A Wagner, R Jokinen, A Hassinen, S Heinonen, S Miettinen, H Peltoniemi, E Pirinen, KH Pietiläine
    Stem Cells International, 2022-04-05;2022(0):3308194.
  5. PRDM16 represses the type I interferon response in adipocytes to promote mitochondrial and thermogenic programing
    Authors: M Kissig, J Ishibashi, MJ Harms, HW Lim, RR Stine, KJ Won, P Seale
    EMBO J., 2017-04-13;0(0):.
  6. Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration
    Authors: TH Ambrosi, A Scialdone, A Graja, S Gohlke, AM Jank, C Bocian, L Woelk, H Fan, DW Logan, A Schürmann, LR Saraiva, TJ Schulz
    Cell Stem Cell, 2017-03-16;20(6):771-784.e6.
  7. A Pan-ALDH1A Inhibitor Induces Necroptosis in Ovarian Cancer Stem-like Cells
    Authors: Chefetz I, Grimley E, Yang K et al.
    Cell Rep
  8. Adverse bioenergetic effects of N-acyl amino acids in human adipocytes overshadow beneficial mitochondrial uncoupling
    Authors: Herrnhold, M;Hamp, I;Plettenburg, O;Jastroch, M;Keuper, M;
    Redox biology
    Species: Human
    Sample Types: Cell Lysates
    Applications: ICC
  9. Human abdominal subcutaneous-derived active beige adipocytes carrying FTO rs1421085 obesity-risk alleles exert lower thermogenic capacity
    Authors: Vámos A, Arianti R, Vinnai B et al.
    Frontiers in Cell and Developmental Biology
  10. Naringenin and beta -carotene convert human white adipocytes to a beige phenotype and elevate hormone- stimulated lipolysis
    Authors: Ann A. Coulter, Frank L. Greenway, Dachuan Zhang, Sujoy Ghosh, Cathryn R. Coulter, Sarah L. James et al.
    Front Endocrinol (Lausanne)
  11. Impairment of adrenergically-regulated thermogenesis in brown fat of obesity-resistant mice is compensated by non-shivering thermogenesis in skeletal muscle
    Authors: P Janovska, P Zouhar, K Bardova, J Otahal, M Vrbacky, T Mracek, K Adamcova, L Lenkova, J Funda, T Cajka, Z Drahota, S Stanic, AC Rustan, O Horakova, J Houstek, M Rossmeisl, J Kopecky
    Molecular Metabolism, 2023-01-30;69(0):101683.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Regulation of beige adipocyte thermogenesis by the cold-repressed ER protein NNAT
    Authors: KM Choi, CY Ko, SM An, SH Cho, DJ Rowland, JH Kim, A Fasoli, AJ Chaudhari, DM Bers, JC Yoon
    Molecular Metabolism, 2023-01-25;0(0):101679.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. MYPT1-PP1beta phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis
    Authors: H Takahashi, G Yang, T Yoneshiro, Y Abe, R Ito, C Yang, J Nakazono, M Okamoto-Ka, A Uchida, M Arai, H Jin, H Choi, M Tumenjarga, S Xie, J Zhang, H Sagae, Y Zhao, R Yamaguchi, Y Nomura, Y Shimizu, K Yamada, S Yasuda, H Kimura, T Tanaka, Y Wada, T Kodama, H Aburatani, MS Zhu, T Inagaki, TF Osborne, T Kawamura, Y Ishihama, Y Matsumura, J Sakai
    Nature Communications, 2022-09-29;13(1):5715.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Identification of UCP1 and UCP2 as Potential Prognostic Markers in Breast Cancer: A Study Based on Immunohistochemical Analysis and Bioinformatics
    Authors: Xin Yu, Manman Shi, Qi Wu, Wen Wei, Shengrong Sun, Shan Zhu
    Frontiers in Cell and Developmental Biology
  15. Chronic Fatty Acid Depletion Induces Uncoupling Protein 1 (UCP1) Expression to Coordinate Mitochondrial Inducible Proton Leak in a Human-Brown-Adipocyte Model
    Authors: Y Takeda, P Dai
    Cells, 2022-06-27;11(13):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice
    Authors: W Liu, H Zhou, H Wang, Q Zhang, R Zhang, B Willard, C Liu, Z Kang, X Li, X Li
    Nature Communications, 2022-05-18;13(1):2748.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Lipocalin 2 Deficiency Alters Prostaglandin Biosynthesis and mTOR Signaling Regulation of Thermogenesis and Lipid Metabolism in Adipocytes
    Authors: J Deis, TY Lin, T Bushman, X Chen
    Cells, 2022-05-03;11(9):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Capsaicin directly promotes adipocyte browning in the chemical compound-induced brown adipocytes converted from human dermal fibroblasts
    Authors: Y Takeda, P Dai
    Scientific Reports, 2022-04-22;12(1):6612.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Progenitor cells from brown adipose tissue undergo neurogenic differentiation
    Authors: M Jumabay, L Zhang, J Yao, KI Boström
    Scientific Reports, 2022-04-04;12(1):5614.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. Defective brown adipose tissue thermogenesis and impaired glucose metabolism in mice lacking Letmd1
    Authors: KM Choi, JH Kim, X Kong, M Isik, J Zhang, HW Lim, JC Yoon
    Cell Reports, 2021-12-14;37(11):110104.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Regulatory modules of human thermogenic adipocytes: functional genomics of large cohort and Meta-analysis derived marker-genes
    Authors: B B Tóth, Z Barta, ÁB Barta, L Fésüs
    BMC Genomics, 2021-12-11;22(1):886.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Latent TGFbeta-binding proteins regulate UCP1 expression and function via TGFbeta2
    Authors: D Halbgebaue, J Roos, JB Funcke, H Neubauer, BS Hamilton, E Simon, EZ Amri, KM Debatin, M Wabitsch, P Fischer-Po, D Tews
    Molecular Metabolism, 2021-09-01;53(0):101336.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Hepatic AKT orchestrates adipose tissue thermogenesis via FGF21-dependent and -independent mechanisms
    Authors: J Sostre-Col, K Uehara, AE Garcia Whi, MJ Gavin, J Ishibashi, MJ Potthoff, P Seale, PM Titchenell
    Cell Reports, 2021-05-18;35(7):109128.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  24. Secreted frizzled‑related protein 4 promotes brown adipocyte differentiation
    Authors: Hua Guan, Huiyuan Zheng, Jin Zhang, Aoqi Xiang, Yongxin Li, Huadong Zheng et al.
    Experimental and Therapeutic Medicine
  25. Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis
    Authors: A Whitehead, FN Krause, A Moran, ADV MacCannell, JL Scragg, BD McNally, E Boateng, SA Murfitt, S Virtue, J Wright, J Garnham, GR Davies, J Dodgson, JE Schneider, AJ Murray, C Church, A Vidal-Puig, KK Witte, JL Griffin, LD Roberts
    Nature Communications, 2021-03-26;12(1):1905.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Transcriptome analysis reveals brown adipogenic reprogramming in chemical compound-induced brown adipocytes converted from human dermal fibroblasts
    Authors: Y Takeda, T Yoshikawa, P Dai
    Scientific Reports, 2021-03-03;11(1):5061.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Adult mice are unresponsive to AAV8-Gremlin1 gene therapy targeting the liver
    Authors: R Khatib Sha, J M Hoffmann, S Hedjazifar, L Bonnet, R K Baboota, S Heasman, C Church, I Elias, F Bosch, J Boucher, A Hammarsted, U Smith
    PLoS ONE, 2021-02-19;16(2):e0247300.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Kdm2a deficiency in macrophages enhances thermogenesis to protect mice against HFD-induced obesity by enhancing H3K36me2 at the Pparg locus
    Authors: L Chen, J Zhang, Y Zou, F Wang, J Li, F Sun, X Luo, M Zhang, Y Guo, Q Yu, P Yang, Q Zhou, Z Chen, H Zhang, Q Gong, J Zhao, DL Eizirik, Z Zhou, F Xiong, S Zhang, CY Wang
    Cell Death and Differentiation, 2021-01-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. Thermogenic Activation Downregulates High Mitophagy Rate in Human Masked and Mature Beige Adipocytes
    Authors: Szatm�ri-T�th M, Shaw A, Csom�s I et al.
    International Journal of Molecular Sciences
  30. 14-3-3? mediates an alternative, non-thermogenic mechanism in male mice to reduce heat loss and improve cold tolerance
    Authors: K Diallo, S Dussault, C Noll, AF Lopez, A Rivard, AC Carpentier, GE Lim
    Mol Metab, 2020-07-12;41(0):101052.
    Species: Mouse
    Sample Types: Protein
    Applications: ICC
  31. Early B Cell Factor Activity Controls Developmental and Adaptive Thermogenic Gene Programming in Adipocytes
    Authors: AR Angueira, SN Shapira, J Ishibashi, S Sampat, J Sostre-Col, MJ Emmett, PM Titchenell, MA Lazar, HW Lim, P Seale
    Cell Rep, 2020-03-03;30(9):2869-2878.e4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  32. A developed serum-free medium and an optimized chemical cocktail for direct conversion of human dermal fibroblasts into brown adipocytes
    Authors: Y Takeda, P Dai
    Sci Rep, 2020-02-28;10(1):3775.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  33. BMP4 gene therapy enhances insulin sensitivity but not adipose tissue browning in obese mice
    Authors: JM Hoffmann, JR Grünberg, A Hammarsted, T Kroon, TU Greiner, S Maurer, I Elias, V Palsdottir, F Bosch, J Boucher, S Hedjazifar, U Smith
    Mol Metab, 2019-12-17;32(0):15-26.
    Species: Mouse
    Sample Types: Tissue ho
    Applications: Western Blot
  34. A PRDM16-Driven Metabolic Signal from Adipocytes Regulates Precursor Cell Fate
    Authors: Wenshan Wang, Jeff Ishibashi, Sophie Trefely, Mengle Shao, Alexis J. Cowan, Alexander Sakers et al.
    Cell Metabolism
  35. Differentiating SGBS adipocytes respond to PPAR? stimulation, irisin and BMP7 by functional browning and beige characteristics
    Authors: Á Klusóczki, Z Veréb, A Vámos, P Fischer-Po, M Wabitsch, Z Bacso, L Fésüs, E Kristóf
    Sci Rep, 2019-04-09;9(1):5823.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  36. Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes
    Authors: MJ Harms, Q Li, S Lee, C Zhang, B Kull, S Hallen, A Thorell, I Alexanders, CE Hagberg, XR Peng, A Mardinoglu, KL Spalding, J Boucher
    Cell Rep, 2019-04-02;27(1):213-225.e5.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  37. Naringenin Promotes Thermogenic Gene Expression in Human White Adipose Tissue
    Authors: Candida J. Rebello, Frank L. Greenway, Frank H. Lau, Yuan Lin, Jacqueline M. Stephens, William D. Johnson et al.
    Obesity (Silver Spring)
  38. A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome
    Authors: S Su, AR Guntur, DC Nguyen, SS Fakory, CC Doucette, C Leech, H Lotana, M Kelley, J Kohli, J Martino, S Sims-Lucas, L Liaw, C Vary, CJ Rosen, AC Brown
    Cell Rep, 2018-12-11;25(11):3215-3228.e9.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  39. Flow Cytometry of Mouse and Human Adipocytes for the Analysis of Browning and Cellular Heterogeneity
    Authors: CE Hagberg, Q Li, M Kutschke, D Bhowmick, E Kiss, IG Shabalina, MJ Harms, O Shilkova, V Kozina, J Nedergaard, J Boucher, A Thorell, KL Spalding
    Cell Rep, 2018-09-04;24(10):2746-2756.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  40. Renal perivascular adipose tissue: Form and function
    Authors: Carolina Baraldi A. Restini, Alex Ismail, Ramya K. Kumar, Robert Burnett, Hannah Garver, Gregory D. Fink et al.
    Vascular Pharmacology
  41. Water–fat magnetic resonance imaging quantifies relative proportions of brown and white adipose tissues: ex-vivo experiments
    Authors: Jadranka Stojanovska, Carey N. Lumeng, Cameron Griffin, Diego Hernando, Udo Hoffmann, Jonathan W. Haft et al.
    Journal of Medical Imaging
  42. IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity Risk
    Authors: Y Zou, P Lu, J Shi, W Liu, M Yang, S Zhao, N Chen, M Chen, Y Sun, A Gao, Q Chen, Z Zhang, Q Ma, T Ning, X Ying, J Jin, X Deng, B Shen, Y Zhang, B Yuan, S Kauderer, S Liu, J Hong, R Liu, G Ning, W Wang, W Gu, J Wang
    EBioMedicine, 2017-09-13;24(0):64-75.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  43. Both brown adipose tissue and skeletal muscle thermogenesis processes are activated during mild to severe cold adaptation in mice
    Authors: NC Bal, S Singh, FCG Reis, SK Maurya, S Pani, LA Rowland, M Periasamy
    J. Biol. Chem., 2017-08-09;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  44. Direct conversion of human fibroblasts to brown adipocytes by small chemical compounds
    Authors: Y Takeda, Y Harada, T Yoshikawa, P Dai
    Sci Rep, 2017-06-27;7(1):4304.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  45. EBF2 transcriptionally regulates brown adipogenesis via the histone reader DPF3 and the BAF chromatin remodeling complex
    Authors: Suzanne N. Shapira, Hee-Woong Lim, Sona Rajakumari, Alexander P. Sakers, Jeff Ishibashi, Matthew J. Harms et al.
    Genes & Development
  46. Overexpressing the novel autocrine/endocrine adipokine WISP2 induces hyperplasia of the heart, white and brown adipose tissues and prevents insulin resistance
    Authors: John R. Grünberg, Jenny M. Hoffmann, Shahram Hedjazifar, Annika Nerstedt, Lachmi Jenndahl, Johannes Elvin et al.
    Scientific Reports
  47. ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs
    Authors: Michelle H. Lee, Anna G. Goralczyk, Rókus Kriszt, Xiu Min Ang, Cedric Badowski, Ying Li et al.
    Scientific Reports
  48. EBF2 promotes the recruitment of beige adipocytes in white adipose tissue
    Authors: Rachel R. Stine, Suzanne N. Shapira, Hee-Woong Lim, Jeff Ishibashi, Matthew Harms, Kyoung-Jae Won et al.
    Molecular Metabolism
  49. White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
    Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C
    Nat Cell Biol, 2014-12-08;17(1):57-67.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  50. Mitophagy Mediates the Beige to White Transition of Human Primary Subcutaneous Adipocytes Ex Vivo
    Authors: VAmos, A;Shaw, A;Varga, K;CsomOs, I;MocsAr, G;Balajthy, Z;LAnyi, C;Bacso, Z;SzatmAri-TOth, M;KristOf, E;
    Pharmaceuticals (Basel, Switzerland)

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Reviews for Human/Mouse UCP1 Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human/Mouse UCP1 Antibody
By Matthew Emmett on 07/12/2017
Application: WB Sample Tested: Brown adipose tissue,Primary brown adipocytes- cultured,Primary Brown Adipocytes Species: Mouse

Ucp1 antibody utilized for western blot and tested against Ucp1 KO brown adipose tissue.

Emmett, M. J. et al. Histone deacetylase 3 prepares brown adipose tissue for acute thermogenic challenge. Nature 546, 544-548 (2017).

Human/Mouse UCP1 Antibody
By Aaron Brown on 07/11/2017
Application: WB Sample Tested: Brown adipose tissue Species: Human
Western Blot Human/Mouse UCP1 Antibody MAB6158