Human/Mouse THAP11 Antibody

Catalog #: AF5727 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5727
AF5727-SP

Detection of Human THAP11 by Western Blot.

1 Image

All THAP11 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse THAP11 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse THAP11 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human THAP11
Gly226-Met314
Accession # Q96EK4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human THAP11 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Human THAP11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5727) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for THAP11 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: THAP11

THAP11, also known as Ronin, is a 36-44 kDa member of the thanatos-associated protein family. It is ubiquitously expressed and through its repression of c-Myc transcription, blocks cell proliferation. Human THAP11 is 314 amino acids (aa) in length. It contains a DNA-binding THAP zinc-finger domain (aa 5-81), an NLS (aa 98-104), a polyGln region (aa 104-146), an Ala-rich segment (aa 197-221) and a C-terminal coiled-coil region (aa 255-305). THAP11 is phosphorylated on Thr25. Over aa 226-314, human and mouse THAP11 are 100% identical in amino acid sequence.

Long Name

THAP Domain Containing 11

Entrez Gene IDs

57215 (Human); 59016 (Mouse); 307806 (Rat)

Alternate Names

CTG-B43a; CTG-B45d; HRIHFB2206; Ronin; THAP domain containing 11; THAP domain-containing protein 11; THAP11

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse THAP11 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse THAP11 Antibody and earn rewards!

Have you used Human/Mouse THAP11 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review