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Human/Mouse Sulfatase-2/SULF2 Antibody

Catalog #: MAB7087
1 Citation Datasheet / COA / SDS
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MAB7087
MAB7087-SP
Detection of Human Sulfatase‑2/SULF2 by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Sulfatase-2/SULF2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Sulfatase-2/SULF2 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 2B4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human Sulfatase-2/SULF2, used to immunize a SULF2-deficient mouse
Accession # NP_940998
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Sulfatase-2/SULF2 antibody by Western Blot. View Larger

Detection of Human Sulfatase‑2/SULF2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Sulfatase-2/SULF2 Monoclonal Antibody (Catalog # MAB7087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Sulfatase-2/SULF2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sulfatase-2/SULF2

Sulfatase 2 (SULF2) is a 132 kDa calcium-binding glycoprotein that is one of two extracellular sulfatases (SULFs). SULFs degrade cell surface and extracellular matrix heparin sulfate proteoglycans, altering the binding and signaling of factors such as Wnts and FGFs. Dysregulation of SULF2 is implicated in carcinogenesis. A high molecular weight form (250 kDa) is presumed to be a covalent dimer, while smaller forms (60 kDa and 64 kDa) are produced by proteolytic cleavage. A splicing form, isoform b, differs only in non-coding regions. Within the region used as an immunogen, human SULF2 shares approximately 94% amino acid sequence identity with mouse and rat SULF2, while mouse and rat SULF2 share 98% amino acid sequence identity with each other.

References
  1. Lemjabbar-Alaoui, H. et al. (2010) Oncogene 29:635.
Entrez Gene IDs
55959 (Human); 72043 (Mouse); 311642 (Rat)
Alternate Names
DKFZp313E091; EC 3.1.6; EC 3.1.6.-; EC 3.1.6.14; extracellular sulfatase Sulf-2; FLJ44488; FLJ90554; hSulf-2; KIAA1247HSULF-2; MGC126411; SULF2; SULF-2; sulfatase 2; Sulfatase2; Sulfatase-2

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Citation for Human/Mouse Sulfatase-2/SULF2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment
    Authors: P Zizza, R Dinami, M Porru, C Cingolani, E Salvati, A Rizzo, C D'Angelo, E Petti, CA Amoreo, M Mottolese, I Sperduti, A Chambery, R Russo, P Ostano, G Chiorino, G Blandino, A Sacconi, J Cherfils-V, C Leonetti, E Gilson, A Biroccio
    Nucleic Acids Res., 2019-04-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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