Human/Mouse Stathmin-2/STMN2 Antibody Summary
Tyr26-Gly179
Accession # Q93045
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human Stathmin-2/STMN2 antibody by Western Blot. Detection of Human Stathmin-2/STMN2 antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/Stathmin-2_MAB6930_Western_Blot_11152.jpg)
Detection of Human Stathmin‑2/STMN2 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Stathmin-1 and recombinant human Stathmin-2 (20 ng/lane) were included. A specific band was detected for Stathmin-2/STMN2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![](https://resources.rndsystems.com/images/datasheets/antibody/mab6930_human-mouse-stathmin-2-stmn2-mab-clone-684433-western-blot-712022111325.jpg)
Detection of Human, Mouse, and Rat Stathmin‑2/STMN2 by Western Blot. Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line, Mouse Brain, and Rat Brain. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse Stathmin‑2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Stathmin‑2/STMN2 at approximately 21-23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
![Stathmin-2/STMN2 antibody in Human Liver by Immunohistochemistry (IHC-P). Stathmin-2/STMN2 antibody in Human Liver by Immunohistochemistry (IHC-P).](https://resources.rndsystems.com/images/datasheets/antibody/Stathmin-2_MAB6930_Immunohistochemistry_11151.jpg)
Stathmin‑2/STMN2 in Human Liver. Stathmin-2/STMN2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Stathmin-2/STMN2
Stathmin-2 (STMN2), also known as superior cervical ganglion 10 protein (SCG10), is a 19 kDa microtubule-destabilizing protein that is preferentially expressed in neurons. It promotes neuronal proliferation and migration as well as neurite outgrowth. Stathmin-2 contains a membrane attachment region
(aa 1‑26), a regulatory domain (aa 39-96), and an overlapping coiled-coil domain (aa 75-179). It is subject to phosphorylation of multiple serines within the regulatory domain. Within aa 26‑179, human Stathmin-2 shares 100% and 99% aa sequence identity with mouse and rat Stathmin-2, respectively.
Product Datasheets
Citations for Human/Mouse Stathmin-2/STMN2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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CRISPR interference to evaluate modifiers of C9ORF72-mediated toxicity in FTD
Authors: Sarah Pickles, Desiree Zanetti Alepuz, Yuka Koike, Mei Yue, Jimei Tong, Pinghu Liu et al.
Frontiers in Cell and Developmental Biology
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The Stathmin-2 membrane targeting domain is required for axon protection and regulated degradation by DLK signaling
Authors: Thornburg-Suresh, EJC;Richardson, JE;Summers, DW;
The Journal of biological chemistry
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A Preliminary Study of Mild Heat Stress on Inflammasome Activation in Murine Macrophages
Authors: Foster, SL;Dutton, AJ;Yerzhan, A;March, LB;Barry, K;Seehus, CR;Huang, X;Talbot, S;Woolf, CJ;
Cells
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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