Human/Mouse STAT1 p91 Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse STAT1 p91 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for STAT1 p91 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Immunoprecipitation of Human STAT1. STAT1 p91 was immunoprecipitated from 200 µg of cell lysate of HeLa human cervical epithelial carcinoma cell line following incubation with 3 µg Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) overnight at 4 °C. STAT1 p91-antibody complexes were absorbed using Protein G sepharose (Invitrogen, Catalog # 10-1242). Immunoprecipitated STAT1 p91 was detected by Western blot using 1 µg/mL Human STAT1 Monoclonal Antibody (Catalog # MAB14901). View our recommended buffer recipes for immunoprecipitation.
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Western Blot Shows Human STAT1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STAT1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot Shows Human STAT1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa cell line, STAT5b KO HeLa cell line, and STAT6 KO HeLa cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human STAT1 by Simple WesternTM. Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for STAT1 at approximately 84 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT1
STAT1 is a member of the STAT family of cytoplasmic transcription factors that mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. Phosphorylation of STAT1a at Y701 leads to dimerization and translocation to the nucleus to activate gene transcription. Human STAT1 shows 93% and 94% aa identity with mouse and rat STAT1, respectively, over the region used as an immunogen. This region is identical between isoforms STAT1a (91 kDa) and STAT1b (84 kDa).
Product Datasheets
Citations for Human/Mouse STAT1 p91 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade
Authors: JJ Ishizuka, RT Manguso, CK Cheruiyot, K Bi, A Panda, A Iracheta-V, BC Miller, PP Du, KB Yates, J Dubrot, I Buchumensk, DE Comstock, FD Brown, A Ayer, IC Kohnle, HW Pope, MD Zimmer, DR Sen, SK Lane-Retic, EJ Robitschek, GK Griffin, NB Collins, AH Long, JG Doench, D Kozono, EY Levanon, WN Haining
Nature, 2018-12-17;565(7737):43-48.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Mediator Kinase Phosphorylation of STAT1 S727 Promotes Growth of Neoplasms With JAK-STAT Activation
Authors: II Nitulescu, SC Meyer, QJ Wen, JD Crispino, ME Lemieux, RL Levine, HE Pelish, MD Shair
EBioMedicine, 2017-11-21;26(0):112-125.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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