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Human/Mouse SSEA-4 APC-conjugated Antibody

Catalog #: FAB1435A
26 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1435A-100
FAB1435A-025
Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (26)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SSEA-4 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SSEA-4 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 APC-conjugated Monoclonal Antibody (Catalog # FAB1435A, filled histogram) or isotype control antibody (Catalog # IC007A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

References
  1. Shevinsky, L.H. et al. (1982) Cell 30:697.
  2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  4. Draper, J.S. et al. (2002) J. Anat. 200:249.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4; SSEA-4; SSEA-4;stage-specific embryonic antigen 4

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Citations for Human/Mouse SSEA-4 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Deciphering neuronal deficit and protein profile changes in human brain organoids from patients with creatine transporter deficiency
    Authors: Broca-Brisson, L;Harati, R;Disdier, C;Mozner, O;Gaston-Breton, R;Maïza, A;Costa, N;Guyot, AC;Sarkadi, B;Apati, A;Skelton, MR;Madrange, L;Yates, F;Armengaud, J;Hamoudi, R;Mabondzo, A;
    eLife
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Revealing cell populations catching the early stages of human embryo development in naive pluripotent stem cell cultures
    Authors: M Moya-Jódar, A Ullate-Ago, P Barlabé, JR Rodríguez-, G Abizanda, C Barreda, X Carvajal-V, A Vilas-Zorn, JP Romero, L Garate, X Agirre, G Coppiello, F Prósper, XL Aranguren
    Stem Cell Reports, 2022-12-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. MS‐Driven Metabolic Alterations Are Recapitulated in iPSC‐Derived Astrocytes
    Authors: Bruno Ghirotto, Danyllo F. Oliveira, Marcella Cipelli, Paulo J. Basso, Jean de Lima, Cristiane N. S. Breda et al.
    Annals of Neurology
  4. Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region
    Authors: S Balasubram, M Andreani, JG Andrade, T Saha, D Sundaravin, J Garzón, W Zhang, O Popp, SI Hiraga, A Rahjouei, DB Rosen, P Mertins, BT Chait, AD Donaldson, M Di Virgili
    Elife, 2022-04-13;11(0):.
    Species: Human
    Sample Types: Whole Cell
    Applications: IF
  5. Multi-omic rejuvenation of human cells by maturation phase transient reprogramming
    Authors: D Gill, A Parry, F Santos, H Okkenhaug, CD Todd, I Hernando-H, TM Stubbs, I Milagre, W Reik
    Elife, 2022-04-08;11(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  6. Generation of NKX2.5GFP Reporter Human iPSCs and Differentiation Into Functional Cardiac Fibroblasts
    Authors: Leyre López-Muneta, Javier Linares, Oscar Casis, Laura Martínez-Ibáñez, Arantxa González Miqueo, Jaione Bezunartea et al.
    Frontiers in Cell and Developmental Biology
  7. Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research
    Authors: EC Pandolfi, TJ Hunt, S Goldsmith, K Hurlbut, SJ Silber, AT Clark
    Stem Cell Research, 2021-06-24;54(0):102446.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research
    Authors: EC Pandolfi, TJ Hunt, S Goldsmith, K Hurlbut, SJ Silber, AT Clark
    Stem Cell Research, 2021-06-08;54(0):102409.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  9. Running the full human developmental clock in interspecies chimeras using alternative human stem cells with expanded embryonic potential
    Authors: Justin Thomas, Ludovic Zimmerlin, Jeffrey S. Huo, Michael Considine, Leslie Cope, Elias T. Zambidis
    npj Regenerative Medicine
  10. Isolation of spermatogenic cells from the cynomolgus macaque testis with flow cytometry
    Authors: Xianzhong Lau, Pearly Jean Ai Yong, Charles Kang Liang Lou, Prabhakaran Munusamy, Mahesh Sangrithi
    STAR Protocols
  11. Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule
    Authors: P Vijayaraj, A Minasyan, A Durra, S Karumbayar, M Mehrabi, CJ Aros, SD Ahadome, DW Shia, K Chung, JM Sandlin, KF Darmawan, KV Bhatt, CC Manze, MK Paul, DC Wilkinson, W Yan, AT Clark, TM Rickabaugh, WD Wallace, TG Graeber, R Damoiseaux, BN Gomperts
    Cell Rep, 2019-12-10;29(11):3488-3505.e9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research
    Authors: EC Pandolfi, EJ Rojas, E Sosa, JJ Gell, TJ Hunt, S Goldsmith, Y Fan, SJ Silber, AT Clark
    Stem Cell Res, 2019-09-16;40(0):101576.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant-Alzheimer's disease (ASUi005-A) and healthy non-demented control (ASUi006-A)
    Authors: N Brookhouse, P Zhang, R Caselli, JJ Kim, DA Brafman
    Stem Cell Res, 2018-09-21;32(0):145-149.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Generation of hiPSTZ16 (ISMMSi003-A) cell line from normal human foreskin fibroblasts
    Authors: M Dejosez, TP Zwaka
    Stem Cell Res, 2017-12-02;26(0):44-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Establishment of an induced pluripotent stem (iPS) cell line from dermal fibroblasts of an asymptomatic patient with dominant PRPF31 mutation
    Authors: A Terray, V Fort, A Slembrouck, C Nanteau, JA Sahel, S Reichman, I Audo, O Goureau
    Stem Cell Res, 2017-10-07;25(0):26-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Generation of an induced pluripotent stem cell (iPSC) line from a patient with autosomal dominant retinitis pigmentosa due to a mutation in the NR2E3 gene
    Authors: A Terray, A Slembrouck, C Nanteau, C Chondroyer, C Zeitz, JA Sahel, I Audo, S Reichman, O Goureau
    Stem Cell Res, 2017-08-05;24(0):1-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Generation and characterization of human induced pluripotent stem cell (hiPSC) lines from an Alzheimer's disease (ASUi001-A) and non-demented control (ASUi002-A) patient homozygous for the Apolipoprotein e4 (APOE4) risk variant
    Authors: N Brookhouse, P Zhang, R Caselli, JJ Kim, DA Brafman
    Stem Cell Res, 2017-06-08;24(0):160-163.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Prospectively Isolated Human Bone Marrow Cell-Derived MSCs Support Primitive Human CD34-Negative Hematopoietic Stem Cells.
    Authors: Matsuoka Y, Nakatsuka R, Sumide K, Kawamura H, Takahashi M, Fujioka T, Uemura Y, Asano H, Sasaki Y, Inoue M, Ogawa H, Takahashi T, Hino M, Sonoda Y
    Stem Cells, 2015-05-01;33(5):1554-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. JMJD5 regulates cell cycle and pluripotency in human embryonic stem cells.
    Authors: Zhu H, Hu S, Baker J
    Stem Cells, 2014-08-01;32(8):2098-110.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Chemically Defined and Small Molecule-Based Generation of Human Cardiomyocytes
    Authors: Paul W. Burridge, Elena Matsa, Praveen Shukla, Ziliang C. Lin, Jared M. Churko, Antje D. Ebert et al.
    Nature Methods
  21. Human embryonic stem cell-derived mesenchymal stroma cells (hES-MSCs) engraft in vivo and support hematopoiesis without suppressing immune function: implications for off-the shelf ES-MSC therapies.
    Authors: Li O, Tormin A, Sundberg B, Hyllner J, Le Blanc K, Scheding S
    PLoS ONE, 2013-01-29;8(1):e55319.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells
    Authors: Tea Soon Park, Ludovic Zimmerlin, Elias T. Zambidis
    Cytometry Part A
  23. Evaluation of ABCG2 expression in human embryonic stem cells: crossing the same river twice?
    Authors: Sarkadi B, Orban TI, Szakacs G, Varady G, Schamberger A, Erdei Z, Szebenyi K, Homolya L, Apati A
    Stem Cells, 2010-01-01;28(1):174-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. High concentration of synthetic serum, stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic stem cells.
    Authors: Lee JY, Lee JE, Kim DK, Yoon TK, Chung HM, Lee DR
    Fertil. Steril., 2008-11-19;93(3):976-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells.
    Authors: Neganova I, Zhang X, Atkinson S, Lako M
    Oncogene, 2008-09-22;28(1):20-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Partial Disturbance of Microprocessor Function in Human Stem Cells Carrying a Heterozygous Mutation in the DGCR8 Gene
    Authors: Dóra Reé, Ábel Fóthi, Nóra Varga, Orsolya Kolacsek, Tamás I. Orbán, Ágota Apáti
    Genes (Basel)

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Human/Mouse SSEA‑4 APC‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Human embryonic stem cells (H9 from Wicell) Species: Human

hPSC were grown for 20 passages as monolayers on matrigel.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: PBS+.1% FBS
Dilution: 1:20

Flow Cytometry Human/Mouse SSEA‑4 APC‑conjugated Antibody FAB1435A