Human/Mouse SSEA-1 PerCP-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of SSEA‑1 in Undifferentiated D3 Mouse Cell Line by Flow Cytometry. Undifferentiated D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse SSEA-1 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2155C, filled histogram) or isotype control antibody (IC015C, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of SSEA‑1 in Whole blood granulocytes by Flow Cytometry. Whole blood granulocytes were stained with Mouse Anti-Human/Mouse SSEA‑1 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2155C, filled histogram) or isotype control antibody (Catalog # IC015C, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SSEA-1
Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).
- Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
- Fox, N. et al. (1983) Cancer Res. 43:669.
Product Datasheets
Citations for Human/Mouse SSEA-1 PerCP-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Intertumoral heterogeneity in patient-specific drug sensitivities in treatment-na�ve glioblastoma
Authors: E Skaga, E Kulesskiy, A Fayzullin, CJ Sandberg, S Potdar, A Kyttälä, IA Langmoen, A Laakso, E Gaál-Paavo, M Perola, K Wennerberg, EO Vik-Mo
BMC Cancer, 2019-06-25;19(1):628.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells
Authors: Hidenori Akutsu, Masakazu Machida, Seiichi Kanzaki, Tohru Sugawara, Takashi Ohkura, Naoko Nakamura et al.
Regenerative Therapy
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