Home / SSEA-1 / Human/Mouse SSEA-1 Alexa Fluor® 700-conjugated Antibody

Human/Mouse SSEA-1 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB2155N
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2155N-100UG
Detection of SSEA‑1 in D3 Mouse Cell Line by Flow Cytometry.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SSEA-1 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SSEA-1.
Source
Monoclonal Mouse IgM Clone # MC-480
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
F9 mouse teratocarcinoma stem cells
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SSEA-1 antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of SSEA‑1 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse SSEA-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2155N, filled histogram) or isotype control antibody (Catalog # IC015N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-1

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

References
  1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
  2. Fox, N. et al. (1983) Cancer Res. 43:669.
Long Name
Stage-specific Embryonic Antigen-1
Alternate Names
alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1

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Citation for Human/Mouse SSEA-1 Alexa Fluor® 700-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Parallel Single-Cell RNA-Seq and Genetic Recording Reveals Lineage Decisions in Developing Embryoid Bodies
    Authors: IS Kim, J Wu, GJ Rahme, S Battaglia, A Dixit, E Gaskell, H Chen, L Pinello, BE Bernstein
    Cell Rep, 2020-10-06;33(1):108222.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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