Human/Mouse Serum Albumin Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Serum Albumin by Western Blot. Western blot shows lysates of human liver tissue and mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Serum Albumin in HepG2 Human Cell Line. Serum Albumin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human and Mouse Albumin by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human Albumin by Simple WesternTM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line undifferentiated (0) and hepatocyte differentiated for 14 and 20 days, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human Albumin by Simple WesternTM. Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Albumin
Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.
Product Datasheets
Citations for Human/Mouse Serum Albumin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Comparison between genetic and pharmaceutical disruption of Ldlr expression for the development of atherosclerosis
Authors: Diego Gomes, Shari Wang, Leela Goodspeed, Katherine E. Turk, Tomasz Wietecha, Yongjun Liu et al.
Journal of Lipid Research
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Inhibition of acyl-CoA binding protein (ACBP) by means of a GABAAR?2-derived peptide
Authors: Anagnostopoulos, G;Saavedra, E;Lambertucci, F;Motiño, O;Dimitrov, J;Roiz-Valle, D;Quesada, V;Alvarez-Valadez, K;Chen, H;Sauvat, A;Rong, Y;Nogueira-Recalde, U;Li, S;Montégut, L;Djavaheri-Mergny, M;Castedo, M;Lopez-Otin, C;Maiuri, MC;Martins, I;Kroemer, G;
Cell death & disease
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Fibrillin-1 Regulates Arteriole Integrity in the Retina
Authors: F Alonso, L Li, I Fremaux, DP Reinhardt, E Génot
Biomolecules, 2022-09-20;12(10):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Blocking CHOP-dependent TXNIP shuttling to mitochondria attenuates albuminuria and mitigates kidney injury in nephrotic syndrome
Authors: SJ Park, Y Kim, C Li, J Suh, J Sivapackia, TM Goncalves, G Jarad, G Zhao, F Urano, V Sharma, YM Chen
Proceedings of the National Academy of Sciences of the United States of America, 2022-08-22;119(35):e2116505119.
Species: Mouse, Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC -
Retinol from hepatic stellate cells via STRA6 induces lipogenesis on hepatocytes during fibrosis
Authors: I Hwang, EJ Lee, H Park, D Moon, HS Kim
Cell & bioscience, 2021-01-06;11(1):3.
Species: Human, Mouse, Transgenic Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Opposing impacts on healthspan and longevity by limiting dietary selenium in telomere dysfunctional mice
Aging Cell, 2016-09-21;0(0):.
Species: Mouse
Sample Types: Plasma
Applications: Functional Assay -
Plasminogen deficiency does not prevent sodium retention in a genetic mouse model of experimental nephrotic syndrome
Authors: Mengyun Xiao, Bernhard N. Bohnert, Hande Aypek, Oliver Kretz, Florian Grahammer, Ute Aukschun et al.
Acta Physiol (Oxf)
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