Human/Mouse/Rat Vimentin APC-conjugated Antibody Summary
Ser2-Glu466
Accession # P08670
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Vimentin in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Rat Anti-Human/Mouse/Rat Vimentin APC-conjugated Monoclonal Antibody (Catalog # IC2105A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Vimentin
Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1-3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine, and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9-11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).
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- Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.
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- Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.
- Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.
- Li, Q-F. et al. (2006) J. Biol. Chem. 281:34716.
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- McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.
- Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.
- Ivaska, J. et al. (2005) EMBO J. 24:3834.
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- Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.
- Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.
- Garg, A. et al. (2006) J. Immunol. 177:6192.
Product Datasheets
Citations for Human/Mouse/Rat Vimentin APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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A bilirubin-derived nanomedicine attenuates the pathological cascade of pulmonary fibrosis
Authors: Hyeongseop Keum, Dohyeon Kim, Jinjoo Kim, Tae Woo Kim, Chang-Hee Whang, Wonsik Jung et al.
Biomaterials
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Orthotopic model of pancreatic cancer using CD34+ humanized mice and generation of tumor organoids from humanized tumors
Authors: Hye Jeong, J;Park, S;Lee, S;Kim, Y;Kyong Shim, I;Jeong, SY;Kyung Choi, E;Kim, J;Jun, E;
International immunopharmacology
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Rhodopsin-positive cell production by intravitreal injection of small molecule compounds in mouse models of retinal degeneration
Authors: Y Fujii, M Arima, Y Murakami, KH Sonoda
PLoS ONE, 2023-02-23;18(2):e0282174.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
RANKL up-regulated by progesterone aggravates lipopolysaccharide-induced acute lung injury during pregnancy
Authors: ZZ Lai, WJ Zhou, JW Shi, YH Meng, JN Wu, JF Ye, T Peng, CE Xu, MQ Li
Journal of reproductive immunology, 2022-12-16;155(0):103788.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
A disease-driver population within interstitial cells of human calcific aortic valves identified via single-cell and proteomic profiling
Authors: JL Decano, Y Iwamoto, S Goto, JY Lee, JT Matamalas, A Halu, M Blaser, LH Lee, B Pieper, S Chelvanamb, J Silva-Nico, F Bartoli-Le, H Higashi, H Shibata, P Vyas, J Wang, E Gostjeva, SC Body, SA Singh, M Aikawa, E Aikawa
Cell Reports, 2022-04-12;39(2):110685.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
New therapeutic opportunities from dissecting the pre-B leukemia bone marrow microenvironment
Authors: LC Cheung, J Tickner, AM Hughes, P Skut, M Howlett, B Foley, J Oommen, JE Wells, B He, S Singh, GA Chua, J Ford, CG Mullighan, RS Kotecha, UR Kees
Leukemia, 2018-05-08;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
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Would you expect FAB210 and IC210 to give different intracellular staining results and why?
Yes. FAB210 was developed to detect cells that are expressing cell surface TNF-alpha and the antibody is able to recognize cells that have bound TNF-alpha on their surface through thier receptor and membrane bound forms. IC210 on the other hand detects the cytoplasmic form o fthe TNF-alpha, that is cells that are actively secreting TNF-alpha intracellularly. If FAB210 is used for intracellular staining, there is a chance that antibody will report addivitve staining of cell surface TNF-alpha as well as intracellular TNF-alpha upon permeabilization.
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