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Human/Mouse/Rat Vimentin Antibody

Catalog #: AF2105
37 Citations 7 Reviews Datasheet / COA / SDS
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AF2105
AF2105-SP
Detection of Human, Mouse, and Rat Vimentin by Western Blot.
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Citations (37)
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Reviews (7)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Vimentin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Vimentin in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Vimentin
Ser2-Glu466
Accession # P08670
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Knockout Validated
Vimentin is specifically detected in K562 human chronic myelogenous leukemia cell line but is not detectable in Vimentin knockout K562 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Vimentin antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Vimentin by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MEF mouse embryonic feeder cells, RAW 264.7 mouse monocyte/macrophage cell line, NR8383 rat alveolar macrophage cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry Vimentin antibody in Human Skin by Immunohistochemistry (IHC-P). View Larger

Vimentin in Human Skin. Vimentin was detected in immersion fixed paraffin-embedded sections of human skin using 10 µg/mL Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry Vimentin antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Vimentin in HeLa Human Cell Line. Vimentin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to intermediate filaments in cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human Vimentin antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Vimentin by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Vimentin at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Knockout Validated Vimentin Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line. View Larger

Vimentin Specificity is Shown by Immunocytochemistry in Knockout Cell Line. Vimentin was detected in immersion fixed K562 human chronic myelogenous leukemia cell line but is not detected in Vimentin knockout (KO) K562 Human Cell Line cell line using Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our Fluorescent ICC Staining of Non-adherent Cells protocol.

Knockout Validated Western Blot Shows Human Vimentin Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Vimentin Specificity by Using Knockout Cell Line. Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and Vimentin knockout K562 cell line (KO). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in knockout K562 cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Western Blot Detection of Human Vimentin by Western Blot View Larger

Detection of Human Vimentin by Western Blot TGF-beta 1, -beta 2 and -beta 3 reduce lymphatic marker expression in LECs.Primary human LECs were treated with 10, 20 or 30 ng/ml TGF-beta 1, -beta 2 and -beta 3 for 72 hours (a) or 100 hours (b). Untreated cells served as a control. Lysates were prepared and analysed by Western blot using antibodies specific for Lyve-1, Prox-1, VEGFR-3 or vimentin. Vinculin served as loading control. The experiment was performed twice with equivalent results. For densitometry evaluation, protein bands were analysed using the software ImageJ. Bands for the Prox-1, Lyve-1, vimentin and VEGFR-3 proteins were normalized to the corresponding loading control and are displayed as the expression level relative to the untreated control samples. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0162221), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human Vimentin by Western Blot View Larger

Detection of Human Vimentin by Western Blot Extracellular vesicles impact multiple signaling elements of the glioma stem cell program.a Mass spectrometry quantification (n = 3) for the presence of MMPs in HUVEC-EVs (EEVs), proneural-GSC157 cells, GSC157 cells treated with CMO and GSC157 cells treated with HUVEC-CM (CME). Comparisons are made relative to untreated GSC157 cells (b, c). MMP activity of GSC157 EVs, HUVEC-EVs and HBEC5i EVs over 1 h (n = 3; b) and cumulatively (n = 3; c). d Cumulative MMP activity for GSC157 cells, GSC157 cells treated with their own EVs (OEVs), HBEC5i EVs and HUVEC-EVs over 1 h (n = 3). e Cumulative MMP activity of GSC157 cells treated with HBEC5i-EVs and either DMSO control or MMP inhibitor, BB94 (n = 3). f Expression of NICD following treatment of proneural-GSC157 cells with OEVs or EEVs in the absence or presence of MMP inhibitors, AG3340 and BB94. g DAVID analysis of the top pathways enriched in proneural-GSC157 cells treated with CME (HUVEC-conditioned media). h Expression of proneural-(NICD) and mesenchymal-(VIM) hallmarks after pharmacological inhibition of: NF kappa B pathway (Bay11-7082), Wnt pathway (LGK974) and TGF beta pathway (LY2157299). i Immunocytochemistry and quantification of phospho-P65 (p-P65) after treatment of GSC157 cells with EEVs (HUVEC-EVs). Densitometry quantifications of p-P65 (j) and total P65 (k) in GSC157 cells treated with HBEC5i-EVs (EEVs) relative to OEVs (n = 3). l Expression of activated (phospho) and total p65 in the presence of Bay11-7082 in GSC157s. m Expression of activated (phospho) and total p65 in the presence of AG3340 and BB94 in GSC157 cells exposed to EEVs. n Quantification of wound healing assay for OEV, EEV or EEVs+Bay 11-7082 treated proneural-GSC157 cells. o Schematic of the different donor EVs (OEVs and EEVs) competing for being taken up by proneural-GSC157. p Either 5,000 or 50,000 GSC157 cells exposed to a fixed amount of 30μg of HBEC5i-EVs (EEVs). q Expression of NICD in the presence of OEVs, HUVEC-EVs (EEVs), OEV:EEV (1:1) and OEV:EEV (2:1). NICD Notch intracellular domain, CMO own conditioned meida, CME endothelial conditioned media, EV extracellular vesicles, OEVs own EVs, EEVs endothelial cell derived EVs, MMP matrix metaloprotinase; HUVEC human umbilical vein endothelial cells, HBEC5i immortalized human brain brain endothelial cells. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/36123372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human Vimentin by Western Blot View Larger

Detection of Human Vimentin by Western Blot NRF1 and/or E2 re-programming contributed to the overexpression of pluripotency and epithelial-mesenchymal transition (EMT) markers and differentiation of tumor initiating breast cancer cells to other-types of cells. Experimental conditions were the same as Figure 1. (A) Immunofluorescent and flow cytometry detections of MCF10A (T) tumor initiating pluripotent stem cells markers (SOX2, Oct4, and Nanog) in transformed breast epithelial cells of MCF10A [MCF-10A (T)] overexpressing vector (control) or NRF1 treated with DMSO and E2. (B) Immunofluorescent and flow cytometry detections of pluripotent stem cells markers (SOX2 and Oct4) in transformed breast epithelial cells of MCF10A [MCF-10A (T)] overexpressing vector (control) or NRF1 treated with DMSO and E2. (C) Immunofluorescent and Western detections of the expression of EMT markers E-cadherin, N-cadherin, and vimentin in transformed breast epithelial cells of MCF10A [MCF-10A (T)] overexpressing vector (control) or NRF1 treated with DMSO and E2. (D) Differentiation of NRF1 tumor initiating stem cells to chondrocytes, neurons, and smooth muscle cells compared to the vector. The vector did not show any differentiation to other cells. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30486409), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vimentin

Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1‑3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97‑98% aa identity with mouse, rat, ovine, bovine and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10‑12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9‑11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).

References
  1. Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383.
  2. Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.
  3. Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614.
  4. Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.
  5. Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.
  6. Li, Q.-F. et al. (2006) J. Biol. Chem. 281:34716.
  7. Esue, O. et al. (2006) J. Biol. Chem. 281:30393.
  8. Styers, M.L. et al. (2005) Traffic 6:359.
  9. McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.
  10. Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.
  11. Ivaska, J. et al. (2005) EMBO J. 24:3834.
  12. Byun, Y. et al. (2001) Cell Death Differ. 8:443.
  13. Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.
  14. Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.
  15. Garg, A. et al. (2006) J. Immunol. 177:6192.
Entrez Gene IDs
7431 (Human); 22352 (Mouse); 81818 (Rat)
Alternate Names
epididymis secretory sperm binding protein; FLJ36605; VIM; Vimentin

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Citations for Human/Mouse/Rat Vimentin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Induction of Reactive Bone Stromal Fibroblasts in 3D Models of Prostate Cancer Bone Metastases
    Authors: Windus, LCE;Matigian, N;Avery, VM;
    Biology
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  2. A spatially resolved single cell genomic atlas of the adult human breast
    Authors: Kumar, T;Nee, K;Wei, R;He, S;Nguyen, QH;Bai, S;Blake, K;Gong, Y;Pein, M;Sei, E;Hu, M;Casasent, A;Thennavan, A;Li, J;Tran, T;Chen, K;Nilges, B;Kashikar, N;Braubach, O;Cheikh, BB;Nikulina, N;Chen, H;Teshome, M;Menegaz, B;Javaid, H;Nagi, C;Montalvan, J;Tifrea, DF;Edwards, R;Lin, E;Parajuli, R;Winocour, S;Thompson, A;Lim, B;Lawson, DA;Kessenbrock, K;Navin, N;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Expression of Connexins 37, 40 and 45, Pannexin 1 and Vimentin in Laryngeal Squamous Cell Carcinomas
    Authors: I Mizdrak, M Mizdrak, A Racetin, B Boškovi?, B Benzon, MG Durdov, K Vukojevi?, N Filipovi?
    Genes, 2023-02-09;14(2):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation
    Authors: J Sun, S Osenberg, A Irwin, LH Ma, N Lee, Y Xiang, F Li, YW Wan, IH Park, M Maletic-Sa, N Ballas
    Cell Reports, 2023-01-05;42(1):111942.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
    Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
    Cell Reports, 2022-09-27;40(13):111423.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells
    Authors: L Adnani, J Kassouf, B Meehan, C Spinelli, N Tawil, I Nakano, J Rak
    Nature Communications, 2022-09-19;13(1):5494.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. The zinc finger transcription factor Sall1 is required for the early developmental transition of microglia in mouse embryos
    Authors: Earl Parker Scott, Emma Breyak, Ryuichi Nishinakamura, Yasushi Nakagawa
    Glia
  8. A novel lineage of osteoprogenitor cells with dual epithelial and mesenchymal properties govern maxillofacial bone homeostasis and regeneration after MSFL
    Authors: Y Weng, H Wang, D Wu, S Xu, X Chen, J Huang, Y Feng, L Li, Z Wang
    Cell Research, 2022-07-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Charting human development using a multi-endodermal organ atlas and organoid models
    Authors: Qianhui Yu, Umut Kilik, Emily M. Holloway, Yu-Hwai Tsai, Christoph Harmel, Angeline Wu et al.
    Cell
  10. A comprehensive library of human transcription factors for cell fate engineering
    Authors: AHM Ng, P Khoshakhla, JE Rojo Arias, G Pasquini, K Wang, A Swiersy, SL Shipman, E Appleton, K Kiaee, RE Kohman, A Vernet, M Dysart, K Leeper, W Saylor, JY Huang, A Graveline, J Taipale, DE Hill, M Vidal, JM Melero-Mar, V Busskamp, GM Church
    Nature Biotechnology, 2020-11-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Dysregulation of tryptophan catabolism at the host-skin microbiota interface in hidradenitis suppurativa
    Authors: Laure Guenin-Macé, Jean-David Morel, Jean-Marc Doisne, Angèle Schiavo, Lysiane Boulet, Véronique Mayau et al.
    JCI Insight
  12. miR‑205 suppresses cell migration, invasion and EMT of colon cancer by targeting mouse double minute 4
    Authors: Yujing Fan, Kuanyu Wang
    Molecular Medicine Reports
  13. Autophagy promotes citrullination of VIM (vimentin) and its interaction with major histocompatibility complex class II in synovial fibroblasts
    Authors: E Sugawara, M Kato, Y Kudo, W Lee, R Hisada, Y Fujieda, K Oku, T Bohgaki, O Amengual, S Yasuda, T Onodera, S Hatakeyama, T Atsumi
    Autophagy, 2019-09-08;0(0):1-10.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  14. Nuclear Respiratory Factor 1 Acting as an Oncoprotein Drives Estrogen-Induced Breast Carcinogenesis
    Authors: Jayanta K. Das, Quentin Felty, Robert Poppiti, Robert M. Jackson, Deodutta Roy
    Cells
  15. Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/??catenin pathway
    Authors: S Zeng, B Zhu, J Zeng, W Wu, C Jiang
    Mol Med Rep, 2018-10-17;0(0):.
  16. Dexamethasone mediates pancreatic cancer progression by glucocorticoid receptor, TGF? and JNK/AP-1
    Authors: L Liu, E Aleksandro, F Schönsiege, D Gröner, N Bauer, CC Nwaeburu, Z Zhao, J Gladkich, T Hoppe-Tich, E Yefenof, T Hackert, O Strobel, I Herr
    Cell Death Dis, 2017-10-05;8(10):e3064.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium.
    Authors: Lynne A Murray, Rebecca Dunmore, Ana Camelo, Carla A Da Silva, Malin J Gustavsso, David M Habiel, Tillie L Hackett, Cory M Hogaboam, Matthew A Sleeman, Darryl A Knight
    Respiratory Research, 2017-05-03;0(0):1465-993X.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Morph-X-Select: Morphology-based tissue aptamer selection for ovarian cancer biomarker discovery
    Authors: David G Gorenstein
    BioTechniques, 2016-11-01;61(5):249-259.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  19. TGF-?1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT)
    PLoS ONE, 2016-09-02;11(9):e0162221.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Targeting CXCR4 by a selective peptide antagonist modulates tumor microenvironment and microglia reactivity in a human glioblastoma model.
    Authors: Mercurio L, Ajmone-Cat M, Cecchetti S, Ricci A, Bozzuto G, Molinari A, Manni I, Pollo B, Scala S, Carpinelli G, Minghetti L
    J Exp Clin Cancer Res, 2016-03-25;35(0):55.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC
  21. Differentiation of Human Induced-Pluripotent Stem Cells into Smooth-Muscle Cells: Two Novel Protocols.
    Authors: Yang L, Geng Z, Nickel T, Johnson C, Gao L, Dutton J, Hou C, Zhang J
    PLoS ONE, 2016-01-15;11(1):e0147155.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  22. Establishment and characterization of an immortalized human hepatic stellate cell line for applications in co-culturing with immortalized human hepatocytes.
    Authors: Pan X, Wang Y, Yu X, Li J, Zhou N, Du W, Zhang Y, Cao H, Zhu D, Chen Y, Li L
    Int J Med Sci, 2015-02-08;12(3):248-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Derivation and High Engraftment of Patient-Specific Cardiomyocyte Sheet Using Induced Pluripotent Stem Cells Generated From Adult Cardiac Fibroblast
    Authors: Liying Zhang, Jing Guo, Pengyuan Zhang, Qiang Xiong, Steven C. Wu, Lily Xia et al.
    Circulation: Heart Failure
  24. The mosaic of "seronegative" antiphospholipid syndrome.
    Authors: Conti F, Capozzi A, Truglia S, Lococo E, Longo A, Misasi R, Alessandri C, Valesini G, Sorice M
    J Immunol Res, 2014-03-17;2014(0):389601.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  25. Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein.
    Authors: Teo, Catherin, Chu, Justin J
    J Virol, 2013-11-27;88(4):1897-913.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer.
    Authors: Windus L, Kiss D, Glover T, Avery V
    Exp Cell Res, 2012-07-27;318(19):2507-19.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  27. Angiotensin II Contributes to Renal Fibrosis Independently of Notch Pathway Activation.
    Authors: Lavoz C, Rodrigues-Diez R, Benito-Martin A, Rayego-Mateos S, Rodrigues-Diez RR, Alique M, Ortiz A, Mezzano S, Egido J, Ruiz-Ortega M
    PLoS ONE, 2012-07-09;7(7):e40490.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  28. Fibroblast activation protein regulates tumor-associated fibroblasts and epithelial ovarian cancer cells.
    Authors: Lai D, Ma L, Wang F
    Int. J. Oncol., 2012-05-14;41(2):541-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. Glycogene expression alterations associated with pancreatic cancer epithelial-mesenchymal transition in complementary model systems.
    Authors: Maupin KA, Sinha A, Eugster E, Miller J, Ross J, Paulino V, Keshamouni VG, Tran N, Berens M, Webb C, Haab BB
    PLoS ONE, 2010-09-27;5(9):e13002.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Vimentin/cardiolipin complex as a new antigenic target of the antiphospholipid syndrome.
    Authors: Ortona E, Capozzi A, Colasanti T, Conti F, Alessandri C, Longo A, Garofalo T, Margutti P, Misasi R, Khamashta MA, Hughes GR, Valesini G, Sorice M
    Blood, 2010-07-15;116(16):2960-7.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development, Immunoprecipitation
  31. Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension.
    Authors: Firth AL, Yao W, Ogawa A, Madani MM, Lin GY, Yuan JX
    Am. J. Physiol., Cell Physiol., 2010-02-24;298(5):C1217-25.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  32. Induction of epithelial-mesenchymal transition in primary airway epithelial cells from patients with asthma by transforming growth factor-beta1.
    Authors: Hackett TL, Warner SM, Stefanowicz D, Shaheen F, Pechkovsky DV, Murray LA, Argentieri R, Kicic A, Stick SM, Bai TR, Knight DA
    Am. J. Respir. Crit. Care Med., 2009-04-30;180(2):122-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. Morphogenesis and biological significance of spindle cell transformation in a spindle cell carcinoma.
    Authors: Kim EJ, Che ZM, Park YJ, Hwang YS, Kim KY, Jung da W, Jeon NK, Choi YW, Lee EJ, Kim J
    Cancer Lett., 2008-11-08;275(1):61-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  34. Single-cell RNA sequencing reveals dysregulated fibroblast subclusters in prurigo nodularis
    Authors: JR Patel, MZ Joel, KK Lee, A Kambala, H Cornman, O Oladipo, M Taylor, J Deng, V Parthasara, K Cravero, M Marani, R Zhao, S Sankararam, R Li, T Pritchard, V Rebecca, MM Kwatra, W Jin Ho, X Dong, S Kang, SG Kwatra
    bioRxiv : the preprint server for biology, 2023-02-03;0(0):.
  35. Placenta-specific protein 1 promotes cell proliferation and invasion in non-small cell lung cancer
    Authors: L Yang, TQ Zha, X He, L Chen, Q Zhu, WB Wu, FQ Nie, Q Wang, CS Zang, ML Zhang, J He, W Li, W Jiang, KH Lu
    Oncol. Rep., 2017-11-09;39(1):53-60.
  36. BRMS1L suppresses breast cancer metastasis by inducing epigenetic silence of FZD10.
    Authors: Gong C, Qu S, Lv Xb et al.
    Nat Commun.
  37. Megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1) promotes glioblastoma cell invasion in the brain microenvironment
    Authors: Lattier JM, De A, Chen Z et al.
    Oncogene

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Reviews for Human/Mouse/Rat Vimentin Antibody

Average Rating: 3.7 (Based on 7 Reviews)

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Human/Mouse/Rat Vimentin Antibody
By Anonymous on 12/20/2022
Application: WB Sample Tested: HepG2 human hepatocellular carcinoma cell line Species: Human
Human/Mouse/Rat Vimentin Antibody
By Anonymous on 07/02/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Cancer Tissue Species: Mouse
Human/Mouse/Rat Vimentin Antibody
By Anonymous on 02/20/2021
Application: IHC Sample Tested: Pancreatic cancer tissue Species: Mouse
Immunohistochemistry Human/Mouse/Rat Vimentin Antibody AF2105
Human Vimentin Antibody
By Anonymous on 06/27/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Pancreas tissue Species: Mouse
Human Vimentin Antibody
By simon liu on 12/19/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Bladder tissue Species: Human

Technical Service will be following up

The customer followed up reporting the poor staining may have been caused by storing the product outside of our guarantee.

Immunocytochemistry/Immunofluorescence Human Vimentin Antibody AF2105
Human Vimentin Antibody
By Anonymous on 12/10/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Kidney cancer tissue Species: Human
Immunocytochemistry/Immunofluorescence Human Vimentin Antibody AF2105
Human Vimentin Antibody
By Juan Zanin on 07/22/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Cerebellum tissue Species: Mouse

P7 MOUSE CEREBELLUM. Radial Glia

Immunocytochemistry/Immunofluorescence Human Vimentin Antibody AF2105