Human/Mouse/Rat VAMP-2 Antibody Summary
Met1-Lys94
Accession # P63027
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human, Mouse, and Rat VAMP-2 antibody by Western Blot. Detection of Human, Mouse, and Rat VAMP-2 antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/VAMP-2_AF5136_Western_Blot_12279.jpg)
Detection of Human, Mouse, and Rat VAMP‑2 by Western Blot. Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line, SH-SY5Y human neuroblastoma cell line, mouse brain tissue, and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat VAMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5136) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP-2 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
![VAMP-2 antibody in Rat Spinal Cord by Immunohistochemistry (IHC-Fr). VAMP-2 antibody in Rat Spinal Cord by Immunohistochemistry (IHC-Fr).](https://resources.rndsystems.com/images/datasheets/antibody/VAMP2_AF5136_Immunohistochemistry_13007.jpg)
VAMP-2 in Rat Spinal Cord. Vesicle-Associate Membrane Protein 2 (VAMP-2) was detected in perfusion-fixed frozen sections of rat spinal cord using a Goat Anti-Human/Mouse/Rat VAMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5136). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # NL001). The cell nuclei were counterstained with DAPI (blue). VAMP-2 immunoreactivity was localized to synaptic vesicles. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VAMP-2
VAMP-2 (vesicle associated membrane protein 2; also synaptobrevin-2) is a 13 kDa member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116 amino acids (aa) in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa 31‑91), and a membrane-anchor domain (aa 95‑114). Over aa 1‑94, human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse/Rat VAMP-2 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat VAMP-2 Antibody and earn rewards!
Have you used Human/Mouse/Rat VAMP-2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image