Human/Mouse/Rat Thymosin beta 10 Antibody

Catalog #: AF6429 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6429
AF6429-SP

Detection of Human, Mouse, and Rat Thymosin beta 10 by Western Blot.

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Human/Mouse/Rat Thymosin beta 10 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Thymosin beta 10 in direct ELISAs. Detects human, mouse, and rat Thymosin beta 10 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Thymosin beta 4 and rhThymosin beta 16 is observed.

Source

Polyclonal Sheep IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Thymosin beta 10 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, NTera-2 human testicular embryonic carcinoma cell line, C6 rat glioma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Thymosin beta 10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6429) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thymosin beta 10 at approximately 9 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Thymosin beta 10

Thymosin beta 10 (T beta 10) is a 6.5 kDa member of the beta -thymosin family of molecules. Members of this family range from 41-44 amino acids (aa) in length, and possess an isoelectric point that lies between pH 5.0-7.0. Multiple cell types produce T beta 10, either constitutively, or after stimulation. They include monocytes/macrophages, endothelial cells, thyroid epithelium, spermatids, fetal cerebellar Purkinje cells plus renal tubule epithelium, and multiple tumor types. T beta 10 is principally an intracellular molecule. It forms a 1:1 complex with G-actin and blocks F-actin polymerization. This regulates the availablility of actin monomers for filament formation and subsequent cell migration. It is also known to be noncanonically secreted, acting on endothelium and blocking angiogenesis. Mature human T beta 10 is 43 aa in length (aa 2-44) (SwissProt #:P63313). It contains an actin-binding site (aa 18-23), an N-terminal acetylated alanine, four acetylated lysines (#4; 15; 26; 39), and one phosphorylation site at Thr23. Mature T beta 10 is identical to mouse T beta 10 in aa sequence, and it shares 74% aa sequence identity with its human family member T beta 4.

Entrez Gene IDs

9168 (Human); 19240 (Mouse)

Alternate Names

MIG12; migration-inducing gene 12; migration-inducing protein 12; PTMB10; TB10; THYB10; Thymosin beta 10; thymosin beta-10; TMSB10