Human/Mouse/Rat TAFA5/FAM19A5 Antibody

Catalog #: AF5148 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5148
AF5148-SP

Detection of Human/Mouse/Rat TAFA5/FAM19A5 by Western Blot.

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Human/Mouse/Rat TAFA5/FAM19A5 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human and mouse TAFA5/FAM19A5 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TAFA5/FAM19A5
Gln26-Ser125
Accession # NP_056196

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

IMR‑32 human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat TAFA5/FAM19A5 by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat TAFA5/FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TAFA5/FAM19A5 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 10.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAFA5/FAM19A5

TAFA5 (also FAM19A5) is a 14 kDa type I transmembrane protein and member of the FAM19/TAFA family of chemokine-like proteins (1). Human TAFA5 is 132 amino acids (aa) in length. It contains a 15 aa extracellular domain, a 23 aa transmembrane sequence, and a 95 aa cytoplasmic region. Alternate splicing produces two additional isoforms. Isoform 2, a secreted form, has a 31 aa substitution for residues 1‑38 in isoform 1. Isoform 3 has an eight aa substitution for residues 1‑87 in isoform1. Human TAFA5 is 100% aa identical to mouse TAFA5 (1). Within the TAFA family, TAFA5 is the most distinct member, while TAFAs 2, 3, and 4 are the most closely related members (1). Real-time PCR analysis indicates that TAFA5 mRNA expression is restricted to the central nervous system (CNS), with the highest level in the basal ganglia and cerebellum (1). The biological functions of TAFA family members are not yet known, but there are a few tentative hypotheses. First, TAFAs may modulate immune responses in the CNS by functioning as brain-specific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). Second, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1‑2). Finally, TAFAs may control axonal sprouting following brain injury (1).

References

Tang, Y.T. et al. (2004) Genomics 83:727.
Benveniste, E. (1998) Cytokine Growth Factor Rev. 9:259.

Long Name

Family with Sequence Similarity 19, Member A5

Entrez Gene IDs

25817 (Human); 106014 (Mouse); 500915 (Rat)

Alternate Names

Chemokine-like protein TAFA-5; FAM19A5; family with sequence similarity 19 (chemokine (C-C motif)-like), member A5; QLLK5208; TAFA protein 5; TAFA5; TAFA-5; UNQ5208