Human/Mouse/Rat SOD2/Mn-SOD Antibody

Detection of Human/Mouse/Rat SOD2/Mn‑SOD by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat SOD2/Mn-SOD Monoclonal Antibody (Catalog # MAB3419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human SOD1, SOD2, and SOD3 (1 ng/lane) were included. A specific band for SOD2/Mn-SOD was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

SOD2/Mn-SOD in MCF‑7 Human Cell Line. SOD2/Mn-SOD was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat SOD2/Mn-SOD Monoclonal Antibody (Catalog # MAB3419) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOD2/Mn‑SOD in HL‑60 Human Cell Line. SOD2/Mn-SOD was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human/Mouse/Rat SOD2/Mn-SOD Monoclonal Antibody (Catalog # MAB3419) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human SOD2/Mn-SOD by Western Blot FAM210B is transported into and is localized in the mitochondria. (a) Schematic representation of FAM210B domains based on the primary structure of FAM210B. (b) Confocal microscopy of HeLa cells transfected with GFP-tagged human FAM210B and RFP-tagged mitochondria. FAM210B-GFP, GFP sequence introduced at the C terminus of FAM210B; FAM210B (MTS)-GFP, the MTS (aa 1–47) of FAM210B was added to the GFP N terminus; FAM210B ( delta MTS)-GFP, GFP was added to the C terminus of MTS (1–47)-deleted CRIF1. Scale bar, 20 mm. (c) Confocal microscopy of HeLa cells transfected with GFP-tagged human FAM210B and RFP-tagged endoplasmic reticulum. (d) Western blotting analysis following subcellular fractionation of GFP-tagged human FAM210B HeLa cells. (e) Western blotting analysis following subcellular fractionation of endogenous in HeLa cells. (f) The mitochondria of HeLa cells were swollen and sonicated to disrupt membranes, washed with alkali buffer (pH 11.5) to detach loosely associated proteins from membranes, and then re-isolated by ultracentrifugation. The supernatant (Supe) and membrane fractions (Pellet) were subjected to western blotting for FAM210B, TOM20, or MnSOD. (g) Mitochondria isolated from HeLa cells were subjected to proteinase K (PK) proteolysis to digest exposed proteins, and detergent (SDS) was used to disrupt both IMMs (inner membrane of mitochondria) and OMMs (outer membrane of mitochondria). The lysates were resolved and subjected to immunoblot analyses. The submitochondrial markers used are Tom20 (OMM), Cyt C (Cytochrome c, intermembrane space), NDUFS1 (IMM), and MnSOD (mitochondrial matrix) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28594398), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human SOD2/Mn-SOD by Western Blot FAM210B is transported into and is localized in the mitochondria. (a) Schematic representation of FAM210B domains based on the primary structure of FAM210B. (b) Confocal microscopy of HeLa cells transfected with GFP-tagged human FAM210B and RFP-tagged mitochondria. FAM210B-GFP, GFP sequence introduced at the C terminus of FAM210B; FAM210B (MTS)-GFP, the MTS (aa 1–47) of FAM210B was added to the GFP N terminus; FAM210B ( delta MTS)-GFP, GFP was added to the C terminus of MTS (1–47)-deleted CRIF1. Scale bar, 20 mm. (c) Confocal microscopy of HeLa cells transfected with GFP-tagged human FAM210B and RFP-tagged endoplasmic reticulum. (d) Western blotting analysis following subcellular fractionation of GFP-tagged human FAM210B HeLa cells. (e) Western blotting analysis following subcellular fractionation of endogenous in HeLa cells. (f) The mitochondria of HeLa cells were swollen and sonicated to disrupt membranes, washed with alkali buffer (pH 11.5) to detach loosely associated proteins from membranes, and then re-isolated by ultracentrifugation. The supernatant (Supe) and membrane fractions (Pellet) were subjected to western blotting for FAM210B, TOM20, or MnSOD. (g) Mitochondria isolated from HeLa cells were subjected to proteinase K (PK) proteolysis to digest exposed proteins, and detergent (SDS) was used to disrupt both IMMs (inner membrane of mitochondria) and OMMs (outer membrane of mitochondria). The lysates were resolved and subjected to immunoblot analyses. The submitochondrial markers used are Tom20 (OMM), Cyt C (Cytochrome c, intermembrane space), NDUFS1 (IMM), and MnSOD (mitochondrial matrix) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28594398), licensed under a CC-BY license. Not internally tested by R&D Systems.