Human/Mouse/Rat SHIP Antibody

Detection of Human SHIP by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line. Gels were loaded with 10 µg (lane 1), 20 µg (lane 2), and 40 µg (lane 3). PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band for SHIP was detected at approximately 148 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

SHIP in Mouse Splenocytes. SHIP was detected in immersion fixed mouse splenocytes using Rat Anti-Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

SHIP Specificity is Shown by Immunocytochemistry in Knockout Cell Line. U-937 WT and SHIP KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Rat Anti-Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) followed by incubation with a goat anti-rat Alexa-fluor 555 coupled secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody concentration used: 1 µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).