Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Name: Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody
Brand: R&D Systems
SKU: MAB1205
Rating: 5 (2 reviews)

Catalog #: MAB1205
8 Citations 2 Reviews Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB1205
MAB1205-SP

Detection of Human and Mouse Phospho-JNK (T183/Y185) by Western Blot.

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Product Details

Citations (8)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat p46 and p54 JNK when dually phosphorylated at sites homologous to T183/Y185 of JNK1 and JNK2, and T221/Y223 of JNK3 in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1006A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing human, rat, and mouse JNK1 T183/Y185 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10-25 µg/mL

See below

Immunocytochemistry

10-35 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Phospho-JNK (T183/Y185) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and 293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm²ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Phospho-JNK (T183/Y185) in HEK293 Human Cell Line. JNK phosphorylated at T183/Y185 was detected in immersion fixed HEK293 human embryonic kidney cell line untreated (lower panel) or treated with UV radiation (upper panel) using Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205) at 25 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI. Filamentous actin was stained with fluorescein-conjugated phalloidin (green). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western

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Detection of Human Phospho-JNK (T183/Y185) by Simple Western^TM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 J/m²ultraviolet light (UV) followed by a 30 minute recovery, loaded at 0.2 mg/mL. A specific band was detected for Phospho-JNK (T183/Y185) at approximately 46 and 56 kDa (as indicated) using 20 μg/ml of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Simple Western

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Detection of Human JNK1/2/3 by Simple Western ACPA inhibits p-Akt, induces p-JNK and affects levels of specific metabolites in NSCLC lines.a Principal component analysis (PCA) score plot: Metabolomics profiling of control and ACPA-treated A549, H1299, H358, and H838 cells. b Changes in variable importance in projection (VIP) values for 19 metabolites in A549 cells. c, d, e Changes in VIP values for 20 metabolites in H1299, H358, and H838 cells. Significantly changed metabolites (*p < 0.05, indicated by arrows) were matched to apoptotic pathways. f, g, h, i Increase and decrease in several metabolites of ACPA-treated A549, H1299, H358, and H838 cells (*p < 0.05). j Simple Western showing total Akt, p-Akt (S473), total JNK46 and JNK54 and p-JNK46 and p-JNK54 (T183/Y185) in A549 cells at 24 hours after treatment with IC50 dose of ACPA. k Relative expression levels of Akt and p-Akt for control and ACPA-treated A549 cells after normalization by total vinculin protein. l Relative expression levels of JNK (46 and 54 kDa) and p-JNK for control and ACPA-treated A549 cells after normalization by total vinculin protein. *p < 0.05, Student’s t-test. All tests were done in quadruplicates. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33431819), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JNK

The c-Jun N-terminal Kinases (JNKs) are part of the MAPK (mitogen-activated protein kinase) system that transmits signals from the extracellular milieu to both the cytoplasm and nucleus of the cell. Following perturbation at the cell membrane, MEKKs/MAP3Ks are initially activated, followed by their activation of MKKs/MAP2Ks, and MKKs activation of MAPKs/MAP(1)Ks. There are three classes of MAPKs: ERKs, p38 Kinases and JNKs. JNKs are 45-55 kDa protein products of three genes which, through alternative splicing, generate up to 10 possible isoforms. The phosphorylation targets for MAPKs vary, but include p53, c-MYC, ATF2 and c-Jun, the latter molecule representing the namesake for the enzyme group. The three human JNKs share approximately 80% aa sequence identity. JNKs from human, mouse and rat all contain a conserved Met-Met-Thr(183)-Pro-Tyr(185)-Val-Val motif that undergoes dual phosphorylation by MMK4 and MMK7 to activate the different JNKs. Activated by environmental stresses and inflammatory cytokines, JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.

Long Name

C-Jun N-terminal Kinase

Alternate Names

c-Jun N-terminal kinase 1; EC 2.7.11; EC 2.7.11.24; JNK; JNK1 alpha protein kinase; JNK1 beta protein kinase; JNK1JNK1A2; JNK-46; JUN N-terminal kinase; MAP kinase 8; MAPK 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8; PRKM8JNK; protein kinase JNK1; SAPK1JNK21B1/2; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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