Human/Mouse/Rat NF-L Antibody Summary
Met1-Asp338
Accession # P07196
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human, Mouse, and Rat NF-L antibody by Western Blot. Detection of Human, Mouse, and Rat NF-L antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/NFL_MAB22161_Western_Blot_22582.jpg)
Detection of Human, Mouse, and Rat NF‑L by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NF-L Monoclonal Antibody (Catalog # MAB22161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NF-L at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
![NF-L antibody in Human Brain by Immunohistochemistry (IHC-P). NF-L antibody in Human Brain by Immunohistochemistry (IHC-P).](https://resources.rndsystems.com/images/datasheets/antibody/NFL_MAB22161_Immunohistochemistry_22022.jpg)
NF‑L in Human Brain. NF-L was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human NF-L Monoclonal Antibody (Catalog # MAB22161) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
![](https://resources.rndsystems.com/images/datasheets/mab22161_human-mouse-rat-nf-l-mab-clone-985912-dual-rnascope-ish-ihc-compatible-2582022144335.jpg)
Detection of NEFL mRNA (red) and protein (green/blue) in Human Cerebellum. NEFL mRNA (red) and protein (green/blue) was detected in formalin-fixed paraffin-embedded tissue sections of human cerebellum. ACD’s Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-NEFL (Catalog # 468671) and mouse anti-human NEFL monoclonal antibody (R&D Systems Catalog #MAB22161) at 5 µg/mL. Tissue was stained using RNAscope® 2.5 HD Detection Reagents-RED (Catalog # 322360), Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and and RNAscope® 2.5 LS Green Accessory Pack (Catalog #322550). Tissue was counterstained with 50% hematoxylin (purple).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NF-L
NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.
Product Datasheets
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