Human/Mouse/Rat mGluR5/1a Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: mGluR5/1a

Rat metabotropic glutamate receptor 5 (mGluR5b; also mGlu5b) is a 150 kDa, 1183 amino acid (aa), 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. Group I receptors include mGluR1 and mGluR5. Both are postsynaptic, associated with Gq-like proteins, mobilize Ca++ from intracellular stores, and regulate neuronal excitability by influencing ion channel activity. Their activities are not necessarily redundant. Although both mobilize intracellular Ca++ stores, mGluR1 also induces Ca++ influx via TRP channels. On T cells, mGluR1 is inducible and promotes cell proliferation, while mGluR5 is constitutive and blocks cell proliferation. mGluR5 has a large glycosylated extracellular domain (ECD) of 558 aa. The ECD either covalently homodimerizes or heterodimerizes with the Ca++-sensor receptor (CaSR). Dimerization creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. There is one alternate splice form of mGluR5b. It shows a 32 aa deletion between aa 876 - 907 in the cytoplasmic region. The rat mGluR5 C-terminal cytoplasmic region is 90% and 97% aa identical to the equivalent regions in human and mouse mGluR5, respectively.

Product Datasheets

Related Research Areas

• Glutamate Receptors
• Microglia Activation
• Neurotransmitter G Protein-Coupled Receptors