Human/Mouse/Rat HNF-3 beta /FoxA2 Antibody

Catalog #: MAB2400 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB2400-100
MAB2400-SP

Detection of Human HNF‑3 beta /FoxA2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat HNF-3 beta /FoxA2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human HNF‑3 beta /FoxA2 in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2141A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human/Mouse/Rat HNF‑3 beta /FoxA2 synthetic peptide
Accession # Q9Y261

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HNF‑3 beta /FoxA2 by Western Blot. Western blot shows lysates of human lung tissue and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat HNF-3 beta /FoxA2 Monoclonal Antibody (Catalog # MAB2400) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HNF-3 beta /FoxA2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of HNF‑3 beta /FoxA2 in Human HepG2 Cells by Flow Cytometry. Human HepG2 cell line was stained with Rabbit Anti-Human/Mouse/Rat HNF-3 beta/FoxA2 Monoclonal Antibody (Catalog # MAB2400, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Flow Cytometry

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Detection of HNF-3 beta /FoxA2 in Human iPS Cells differentiated to Mesoderm by Flow Cytometry. Human iPS cells differentiated to Mesoderm (Catalog # SC030B) were stained with Rabbit Anti-Human/Mouse/Rat HNF-3 beta/FoxA2 Monoclonal Antibody (Catalog # MAB2400, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Immunohistochemistry

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HNF‑3 beta /FoxA2 in Human Liver Cancer Tissue. HNF-3 beta /FoxA2 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human/Mouse/Rat HNF-3 beta /FoxA2 Monoclonal Antibody (Catalog # MAB2400) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HNF-3 beta/FoxA2

HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.

Long Name

Hepatocyte Nuclear Factor-3, beta

Entrez Gene IDs

3170 (Human)

Alternate Names

forkhead box A2; Forkhead box protein A2; FoxA2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; hepatocyte nuclear factor 3-beta; HNF3 beta; HNF-3 beta; HNF-3B; HNF-3-beta; HNF3BTCF-3B; MGC19807; TCF3B; Transcription factor 3B