Human/Mouse/Rat GRP78/HSPA5 Antibody

Catalog # Availability Size / Price Qty
AF4846
AF4846-SP
Detection of Human and Rat GRP78/HSPA5 by Western Blot.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse/Rat GRP78/HSPA5 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat GRP78 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GRP78/HSPA5
Met1-Leu654
Accession # P11021
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Rat GRP78/HSPA5 antibody by Western Blot. View Larger

Detection of Human and Rat GRP78/HSPA5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRP78/HSPA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4846) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRP78/HSPA5 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human GRP78/HSPA5 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human GRP78/HSPA5 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GRP78/HSPA5 at approximately 74 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat GRP78/HSPA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4846) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRP78/HSPA5

GRP78 (Glucose-regulated protein 78 kDa; also BiP and HSPA5) is a 72 kDa member of the heat shock protein 70 family of proteins. Intracellularly, GRP78 is an endoplasmic reticulum chaperone that participates in protein folding; extracellularly, it induces IL-10 production from T cells and interacts with Cripto to block TGF-beta signaling. Human GRP78 precursor is 654 amino acids (aa) in length. It contains an 18 aa signal sequence and a 636 aa mature region that shows a hydantoinase A region (aa 145‑245) and a C-terminal KDEL motif that is present on intracellular GRP78, but absent on secreted GRP78. There is alternative splicing in the signal sequence (aa 1‑10), and multiple single aa substitituion. Over aa 1‑654, human GRP78 is more than 97% aa identical to mouse and rat GRP78.

Long Name
75 KD Glucose Regulated Protein
Entrez Gene IDs
3309 (Human); 14828 (Mouse); 25617 (Rat)
Alternate Names
78 kDa glucose-regulated protein; BIP; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; FLJ26106; GRP78; GRP-78; GRP78BIP; Heat shock 70 kDa protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); HSP70-5; HSPA5; Immunoglobulin heavy chain-binding protein; MIF2

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Citations for Human/Mouse/Rat GRP78/HSPA5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Cooperative treatment effectiveness of ATR and HSP90 inhibition in Ewing's sarcoma cells
    Authors: C Marx, MU Schaarschm, J Kirkpatric, L Marx-Blüme, M Halilovic, M Westermann, D Hoelzer, FB Meyer, Y Geng, K Buder, HM Schadwinke, K Siniuk, S Becker, R Thierbach, JF Beck, J Sonnemann, ZQ Wang
    Cell & bioscience, 2021-03-20;11(1):57.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. XBP1 signalling is essential for alleviating mutant protein aggregation in ER-stress related skeletal disease
    Authors: KA Piróg, EP Dennis, CL Hartley, RM Jackson, J Soul, JM Schwartz, JF Bateman, RP Boot-Handf, MD Briggs
    PLoS Genet., 2019-07-01;15(7):e1008215.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response
    Authors: Paloma Gómez-Fernández, Andoni Urtasun, Adrienne W. Paton, James C. Paton, Francisco Borrego, Devin Dersh et al.
    Frontiers in Immunology
  4. The Role of Autophagy in the Degradation of Misfolded HLA-B27 Heavy Chains
    Authors: F Navid, G Layh-Schmi, KA Sikora, A Cougnoux, RA Colbert
    Arthritis & rheumatology (Hoboken, N.J.), 2018-03-25;0(0):.
    Species: Rat
    Sample Types: Cell Lysate, Whole Cells
    Applications: Immunoprecipitation, Western Blot
  5. The Novel Mechanisms Concerning the Inhibitions of Palmitate-Induced Proinflammatory Factor Releases and Endogenous Cellular Stress with Astaxanthin on MIN6 ?-Cells
    Authors: A Kitahara, K Takahashi, N Morita, T Murashima, H Onuma, Y Sumitani, T Tanaka, T Kondo, T Hosaka, H Ishida
    Mar Drugs, 2017-06-20;15(6):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-kappaappa B signaling induced by tumor necrosis factor-alpha in human A549 lung epithelial cells.
    Authors: Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N
    PLoS ONE, 2014-06-24;9(6):e100787.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß.
    Authors: Oida T, Weiner HL
    J. Immunol., 2010-08-18;185(6):3529-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat GRP78/HSPA5 Antibody

Average Rating: 5 (Based on 1 Review)

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Human/Mouse/Rat GRP78/HSPA5 Antibody
By Anonymous on 10/17/2018
Application: WB Sample Tested: A549 human lung carcinoma cell line,H69AR human small cell lung cancer cell line Species: Human