Human/Mouse/Rat GRB7 Antibody

Catalog #: AF4856 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4856
AF4856-SP

Detection of Human/Mouse/Rat GRB7 by Western Blot.

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Human/Mouse/Rat GRB7 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat GRB7 in Western blots.

Source

Polyclonal Sheep IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat GRB7 by Western Blot. Western blot shows lysates of CCD-1070Sk human foreskin fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRB7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4856) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRB7 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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GRB7 in Human Liver Cancer Tissue. GRB7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Sheep Anti-Human/Mouse/Rat GRB7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4856) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: GRB7

GRB7 (Growth factor receptor-bound protein 7) mediates the coupling of multiple cell surface receptors to downstream signaling pathways in the regulation of various cellular functions, such as proliferation, apoptosis, and cell migration. GRB7 protein consists of several distinctive structural features, including an amino-terminal proline-rich region, a central GM region (for Grb and Mig), and a carboxyl-terminal SH2 domain. GRB7 is phosphorylated by focal adhesion kinase at cell adhesion sites.

Long Name

Growth Factor Receptor-Bound Protein 7

Entrez Gene IDs

2886 (Human); 14786 (Mouse); 84427 (Rat)

Alternate Names

B47; Epidermal growth factor receptor GRB-7; GRB7 adapter protein; GRB7; growth factor receptor-bound protein 7