Human/Mouse/Rat FoxP3 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC3240A
Detection of FoxP3 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (2)
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Human/Mouse/Rat FoxP3 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human FoxP3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human FoxD3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FoxP3
Gln105-Lys200
Accession # Q9BZS1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of FoxP3 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of FoxP3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Mouse/Rat FoxP3 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3240A) and either (A) Mouse Anti-Human CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3791C) or (B) Mouse Anti-Human IL-2 Ra PE-conjugated Monoclonal Antibody (Catalog # FAB1020P). Quadrant markers were set based on control antibody staining (Catalog # IC108A).

Intracellular Staining by Flow Cytometry Detection of FoxP3 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Human/Mouse/Rat FoxP3 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3240A) and either (A) Rat Anti-Mouse CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB554C) or (B) Rat Anti-Mouse IL-2 Ra PE-conjugated Monoclonal Antibody (Catalog # FAB2438P). Quadrant markers were set based on control antibody staining (Catalog # IC108A).

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FoxP3

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells. In human, FoxP3 is found in CD4+, CD8+ and CD4+CD25+ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Long Name
Forkhead Box P3
Entrez Gene IDs
50943 (Human); 20371 (Mouse)
Alternate Names
AIID; AIIDMGC141961; DIETER; forkhead box P3; Forkhead Box Protein P3; FoxP3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; IPEX; JM2; MGC141961; MGC141963; PIDX; PIDXMGC141963; SCURFIN; XPID; XPIDpolyendocrinopathy, enteropathy, X-linked

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Citations for Human/Mouse/Rat FoxP3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Elevated CD39+T-Regulatory Cells and Reduced Levels of Adenosine Indicate a Role for Tolerogenic Signals in the Progression from Moderate to Severe COVID-19
    Authors: Elsaghir, A;El-Sabaa, EMW;Zahran, AM;Mandour, SA;Salama, EH;Aboulfotuh, S;El-Morshedy, RM;Tocci, S;Mandour, AM;Ali, WE;Abdel-Wahid, L;Sayed, IM;El-Mokhtar, MA;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. A small-molecule inhibitor of macrophage migration inhibitory factor for the treatment of inflammatory disease.
    Authors: Kithcart AP, Cox GM, Sielecki T
    FASEB J., 2010-07-12;24(11):4459-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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