Human/Mouse/Rat FLIP Antibody Summary
Met1-Asn200
Accession # O15519
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat FLIP by Western Blot. Western blot shows lysates of PANC-1 human pancreatic carcinoma cell line, L-929 mouse fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat FLIP Monoclonal Antibody (Catalog # MAB8430) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FLIP at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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FLIP in Human Pancreatic Cancer Tissue. FLIP was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human/Mouse/Rat FLIP Monoclonal Antibody (Catalog # MAB8430) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cells in islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FLIP
FLIP (Flice-Like Inhibitory Protein), also known as CFLAR (CASP8 and FADD-Like Apoptosis Regulator), I-FLICE, CASPER, and FLAME-1, is an apoptosis inhibitory protein with architecture similar to that of Caspases-8 and -10. Both of the major FLIP isoforms, the 55 kDa Long (L) and the 27 kDa Short (S), contain two death effector domains (DED). FLIP(L) has a C-terminal Caspase-like protease domain which lacks both a catalytic active site and residues that form a substrate-binding pocket. FLIP(S) and FLIP(L) interact with the adaptor molecule FADD, and potently inhibit apoptosis initiated by Fas Ligand/TNF signaling pathways. Over amino acids 1-200, human FLIP shares 75% sequence identity with mouse and rat FLIP.
Product Datasheets
Citations for Human/Mouse/Rat FLIP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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LILRB3 supports acute myeloid leukemia development and regulates T-cell antitumor immune responses through the TRAF2-cFLIP-NF-?B signaling axis
Authors: Wu G, Xu Y, Schultz RD et al.
Nature Cancer
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Induction of immunosuppressive functions and NF-?B by FLIP in monocytes
Authors: A Fiore, S Ugel, F De Sanctis, S Sandri, G Fracasso, R Trovato, S Sartoris, S Solito, S Mandruzzat, F Vascotto, KL Hippen, G Mondanelli, U Grohmann, G Piro, C Carbone, D Melisi, RT Lawlor, A Scarpa, A Lamolinara, M Iezzi, M Fassan, S Bicciato, BR Blazar, U Sahin, PJ Murray, V Bronte
Nat Commun, 2018-12-05;9(1):5193.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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