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Human/Mouse/Rat FABP3/H-FABP Antibody

Catalog #: AF1678
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1678
AF1678-SP
Detection of Human, Mouse, and Rat FABP3/H‑FABP by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat FABP3/H-FABP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human FABP3/H‑FABP in direct ELISAs and detects human, mouse, and rat FABP3/H-FABP in Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) FABP4 and recombinant human (rh) FABP4 is observed, approximately 15% cross-reactivity with recombinant rat (rr) FABP1, rhFABP1, rhFABP2, rhFABP7, and rhFABP8 is observed, and less than 10% cross-reactivity with rrFABP2 and rmFABP5 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FABP3/H‑FABP
Met1-Ala133
Accession # P05413
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat FABP3/H-FABP antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat FABP3/H‑FABP by Western Blot. Western blot shows lysates of human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FABP3/H-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry FABP3/H-FABP antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

FABP3/H‑FABP in Human Heart. FABP3/H-FABP was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human/Mouse/Rat FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human and Mouse FABP3/H-FABP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse FABP3/H‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP3/H-FABP at approximately 19 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP3/H-FABP

Human FABP3, also known as FABP-Heart (H-FABP), FABP-Muscle (M-FABP) and mammary derived growth inhibitor (MDGI), is a member of the intracellular FABP family. It plays a role in the transport of long chain fatty acids in the cytoplasm.

Long Name
Fatty Acid-Binding Protein 3
Entrez Gene IDs
2170 (Human)
Alternate Names
FABP11; FABP3; fatty acid binding protein 11; fatty acid binding protein 3, muscle and heart (mammary-derived growthinhibitor); Fatty acid-binding protein 3; Fatty acid-binding protein 3, muscle; fatty acid-binding protein, heart; Heart-type fatty acid-binding protein; HFABP; H-FABP; H-FABPM-FABP; Mammary-derived growth inhibitor; MDGI; Muscle fatty acid-binding protein; O-FABP

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Citations for Human/Mouse/Rat FABP3/H-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Expression of fatty acid-binding proteins in adult hippocampal neurogenic niche of postischemic monkeys.
    Authors: Boneva NB, Kaplamadzhiev DB, Sahara S, Kikuchi H, Pyko IV, Kikuchi M, Tonchev AB, Yamashima T
    Hippocampus, 2011-02-01;21(2):162-71.
    Species: Primate - Macaca fuscata (Japanese Macaque)
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  2. Formation of a human-derived fat tissue layer in P(DL)LGA hollow fibre scaffolds for adipocyte tissue engineering.
    Authors: Morgan SM, Ainsworth BJ, Kanczler JM, Babister JC, Chaudhuri JB, Oreffo RO
    Biomaterials, 2009-01-09;30(10):1910-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. The use of small interfering RNAs to inhibit adipocyte differentiation in human preadipocytes and fetal-femur-derived mesenchymal cells.
    Authors: Mirmalek-Sani SH, Oreffo RO
    Exp. Cell Res., 2006-04-17;312(10):1856-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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