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Human/Mouse/Rat Ephrin-B2 Antibody

Catalog #: AF496
47 Citations 2 Reviews Datasheet / COA / SDS
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AF496
AF496-SP
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Detection of Mouse Ephrin‑B2 by Western Blot.
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Citations (47)
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Reviews (2)
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Human/Mouse/Rat Ephrin-B2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects mouse Ephrin-B2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse Ephrin-B1 is observed.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Ephrin‑B2 antibody by Western Blot. View Larger

Detection of Mouse Ephrin‑B2 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Ephrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Ephrin-B2 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry Detection of Ephrin-B2 antibody in SH-SY5Y Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Ephrin‑B2 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat Ephrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunocytochemistry Ephrin-B2 antibody in Rat Hippo-campal Neurons by Immunocytochemistry (ICC). View Larger

Ephrin‑B2 in Rat Hippo-campal Neurons. Ephrin‑B2 was detected in immersion fixed rat hippocampal neurons using 2 µg/mL Goat Anti-Human/Mouse/Rat Ephrin‑B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Ephrin-B2

Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can become tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 amino acids long and are highly conserved, especially the last 33 amino acids. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B2 has been shown to bind EphA4, EphB1, EphB2, EphB3, and EphB4 (2, 3). The extracellular domains of murine and human Ephrin-B2 share 98% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The Ephrin/Eph families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter]. (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Entrez Gene IDs
1948 (Human); 13642 (Mouse); 30219 (Zebrafish)
Alternate Names
EFNB2; ELF-2; ephrin B2; EphrinB2; Ephrin-B2; EPLG5; HTKL; Htk-L; LERK5; LERK-5; NLERK-1

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Citations for Human/Mouse/Rat Ephrin-B2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

47 Citations: Showing 1 - 10
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  1. EphrinB2-EphB4 signalling provides Rho-mediated homeostatic control of lymphatic endothelial cell junction integrity
    Authors: Maike Frye, Simon Stritt, Henrik Ortsäter, Magda Hernandez Vasquez, Mika Kaakinen, Andres Vicente et al.
    eLife
  2. Exercise-induced angiogenesis is dependent on metabolically primed ATF3/4+ endothelial cells
    Authors: Zheng Fan, Guillermo Turiel, Raphaela Ardicoglu, Moheb Ghobrial, Evi Masschelein, Tea Kocijan et al.
    Cell Metabolism
  3. SMAD4 maintains the fluid shear stress set point to protect against arterial-venous malformations
    Authors: Kuheli Banerjee, Yanzhu Lin, Johannes Gahn, Julio Cordero, Purnima Gupta, Islam Mohamed et al.
    Journal of Clinical Investigation
  4. Rapid remodeling of airway vascular architecture at birth.
    Authors: Ni A, Lashnits E, Yao LC et al.
    Dev Dyn
  5. The Value of EphB2 Receptor and Cognate Ephrin Ligands in Prognostic and Predictive Assessments of Human Breast Cancer
    Authors: Abdul Shukkur Ebrahim, Zeyad Hailat, Sudeshna Bandyopadhyay, Daniel Neill, Mustapha Kandouz
    International Journal of Molecular Sciences
  6. Generating human artery and vein cells from pluripotent stem cells highlights the arterial tropism of Nipah and Hendra viruses
    Authors: Lay Teng Ang, Alana T. Nguyen, Kevin J. Liu, Angela Chen, Xiaochen Xiong, Matthew Matthew Curtis et al.
    Cell
  7. Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury
    Authors: Victor H. Guaiquil, Cissy Xiao, Daniel Lara, Greigory Dimailig, Qiang Zhou
    Experimental Eye Research
  8. Activation of EphrinB2 Signaling Promotes Adaptive Venous Remodeling in Murine Arteriovenous Fistulae
    Authors: Tun Wang, Jia Liu, Haiyang Liu, Shin-Rong Lee, Luis Gonzalez, Jolanta Gorecka et al.
    Journal of Surgical Research
  9. Cdk5 controls lymphatic vessel development and function by phosphorylation of Foxc2
    Authors: Johanna Liebl, Siwei Zhang, Markus Moser, Yan Agalarov, Cansaran Saygili Demir, Bianca Hager et al.
    Nature Communications
  10. Ephrin-B2/EphA4 forward signaling is required for regulation of radial migration of cortical neurons in the mouse
    Authors: Yan Hu, Sen Li, Hua Jiang, Ming-Tao Li, Jia-Wei Zhou
    Neuroscience Bulletin
  11. The Midline Axon Crossing Decision Is Regulated through an Activity-Dependent Mechanism by the NMDA Receptor
    Authors: Jingxia Gao, Tamara J. Stevenson, Adam D. Douglass, Joshua P. Barrios, Joshua L. Bonkowsky
    eNeuro
  12. EphrinB2 knockdown in spinal cord astrocytes preserves diaphragm innervation in a mutant SOD1 mouse model of ALS
    Authors: Urban, MW;Charsar, BA;Heinsinger, NM;Markandaiah, SS;Sprimont, L;Zhou, W;Henderson, NT;Ghosh, B;Cain, RE;Trotti, D;Pasinelli, P;Wright, MC;Dalva, MB;Lepore, AC;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Actin cable formation and epidermis-dermis positional relationship during complete skin regeneration
    Authors: K Takaya, K Okabe, A Ishigami, Y Imbe, H Kanazawa, S Sakai, N Aramaki-Ha, K Kishi
    Scientific Reports, 2022-09-23;12(1):15913.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Histochemical examination of blood vessels in murine femora with intermittent PTH administration
    Authors: H Maruoka, S Zhao, H Yoshino, M Abe, T Yamamoto, H Hongo, M Haraguchi-, A Nasoori, H Ishizu, Y Nakajima, M Omaki, T Shimizu, N Iwasaki, PH Luiz de Fr, M Li, T Hasegawa
    Journal of oral biosciences, 2022-05-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture
    Authors: S Yamasaki, A Kuwahara, A Kishino, T Kimura, M Takahashi, M Mandai
    Regenerative Therapy, 2022-01-01;19(0):24-34.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  16. EphrinB2-RhoA upregulation attenuates sympathetic hyperinnervation and decreases the incidence of ventricular arrhythmia after myocardial infarction
    Authors: Y Wang, C Shao, L Qi, J Tan, Y Zhao, M Xue, X Li, W Cheng, X Li, J Yin, Y Shi, Y Wang, K Wang, H Hu, S Yan
    Journal of cardiology, 2021-11-05;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo
    Authors: YW Ge, K Feng, XL Liu, HF Chen, ZY Sun, CF Wang, ZQ Liu, HW Wang, JW Zhang, DG Yu, YQ Mao
    Oxid Med Cell Longev, 2020-06-05;2020(0):1404915.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  18. Digenic inheritance of mutations in EPHA2 and SLC26A4 in Pendred syndrome
    Authors: M Li, SY Nishio, C Naruse, M Riddell, S Sapski, T Katsuno, T Hikita, F Mizapoursh, FM Smith, LT Cooper, MG Lee, M Asano, T Boettger, M Krueger, A Wietelmann, J Graumann, BW Day, AW Boyd, S Offermanns, SI Kitajiri, SI Usami, M Nakayama
    Nat Commun, 2020-03-12;11(1):1343.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development
    Authors: E Harde, L Nicholson, B Furones Cu, D Bissen, S Wigge, S Urban, M Segarra, C Ruiz de Al, A Acker-Palm
    Elife, 2019-12-23;8(0):.
    Species: Mouse
    Sample Types: Tissue Lysates, Whole Tissues
    Applications: IHC, Western Blot
  20. EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells
    Authors: W Shi, Y Wang, J Peng, S Qi, N Vitale, N Kaneda, T Murata, H Luo, J Wu
    J. Biol. Chem., 2019-03-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  21. Inhibition of Ephrin-B2 in brain pericytes decreases cerebral pathological neovascularization in diabetic rats
    Authors: M Coucha, AC Barrett, M Elgebaly, A Ergul, M Abdelsaid
    PLoS ONE, 2019-01-08;14(1):e0210523.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  22. EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF
    Authors: E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi
    EMBO Rep., 2018-04-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1?
    Authors: KM Tsang, JS Hyun, KT Cheng, M Vargas, D Mehta, M Ushio-Fuka, L Zou, KV Pajcini, J Rehman, AB Malik
    Stem Cell Reports, 2017-08-03;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice
    Authors: H Toray, T Hasegawa, N Sakagami, E Tsuchiya, A Kudo, S Zhao, Y Moritani, M Abe, T Yoshida, T Yamamoto, T Yamamoto, K Oda, N Udagawa, PH Luiz de Fr, M Li
    Biomed. Res., 2017-01-01;38(2):123-134.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance
    Nat Commun, 2016-07-29;7(0):12329.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  26. Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
    Authors: Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E
    PLoS Genet, 2015-11-03;11(11):e1005638.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  27. GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model.
    Authors: Dong L, Gao F, Wang X, Miao Y, Wang S, Wu Y, Li F, Wu J, Cheng X, Sun X, Yang X, Wang Z
    J Neurosci, 2015-04-01;35(13):5409-21.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  28. EphrinB2 controls vessel pruning through STAT1-JNK3 signalling.
    Authors: Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G
    Nat Commun, 2015-03-26;6(0):6576.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Proximity Ligation Assay (PLA)
  29. Biomaterial microenvironments to support the generation of new neurons in the adult brain.
    Authors: Conway A, Schaffer D
    Stem Cells, 2014-05-01;32(5):1220-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1.
    Authors: Ji Y, Hwang Y, Mood K, Cho H, Lee H, Winterbottom E, Cousin H, Daar I
    Nat Commun, 2014-03-24;5(0):3516.
    Species: Human, Xenopus
    Sample Types: Cell Lysates, Embryo
    Applications: Immunoprecipitation
  31. EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury.
    Authors: Kida Y, Ieronimakis N, Schrimpf C, Reyes M, Duffield J
    J Am Soc Nephrol, 2013-03-14;24(4):559-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  32. Neuropsin cleaves EphB2 in the amygdala to control anxiety.
    Authors: Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R
    Nature, 2011-04-20;473(7347):372-5.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  33. Hedgehog signaling induces arterial endothelial cell formation by repressing venous cell fate.
    Authors: Williams C, Kim SH, Ni TT, Mitchell L, Ro H, Penn JS, Baldwin SH, Solnica-Krezel L, Zhong TP
    Dev. Biol., 2010-02-26;341(1):196-204.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  34. Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro.
    Authors: He S, Kumar SR, Zhou P, Krasnoperov V, Ryan SJ, Gill PS, Hinton DR
    Invest. Ophthalmol. Vis. Sci., 2009-08-20;51(1):543-52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  35. EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts.
    Authors: Allan EH, Hausler KD, Wei T, Gooi JH, Quinn JM, Crimeen-Irwin B, Pompolo S, Sims NA, Gillespie MT, Onyia JE, Martin TJ
    J. Bone Miner. Res., 2008-08-01;23(8):1170-81.
    Species: Rat
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  36. Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice.
    Authors: Zhang J, Dong H, Wang B, Zhu S, Croy BA
    Biol. Reprod., 2008-05-07;79(3):450-8.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  37. The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
    Authors: Yang NY, Pasquale EB, Owen LB, Ethell IM
    J. Biol. Chem., 2006-08-31;281(43):32574-86.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  38. Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.
    Authors: Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG
    J. Cell. Sci., 2003-05-06;116(0):2461-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  39. Vascular endothelial growth factor induces contralesional corticobulbar plasticity and functional neurological recovery in the ischemic brain
    Authors: Raluca Reitmeir, Ertugrul Kilic, Barbara S. Reinboth, Zeyun Guo, Ayman ElAli, Anil Zechariah et al.
    Acta Neuropathologica
  40. Role of Notch1 in the arterial specification and angiogenic potential of mouse embryonic stem cell-derived endothelial cells
    Authors: Jae Kyung Park, Tae Wook Lee, Eun Kyoung Do, Hye Ji Moon, Jae Ho Kim
    Stem Cell Research & Therapy
  41. Why some tumours trigger neovascularisation and others don’t: the story thus far
    Authors: Omanma Adighibe, Russell D. Leek, Marta Fernandez-Mercado, Jiangting Hu, Cameron Snell, Kevin C. Gatter et al.
    Chinese Journal of Cancer
  42. Ephrin-B2 controls PDGFR-beta internalization and signaling.
    Authors: Nakayama A, Nakayama M, Turner CJ et al.
    Genes Dev
  43. Hsp90 Inhibitors Are Efficacious against Kaposi Sarcoma by Enhancing the Degradation of the Essential Viral Gene LANA, of the Viral Co-Receptor EphA2 as well as Other Client Proteins
    Authors: Wuguo Chen, Sang-Hoon Sin, Kwun Wah Wen, Blossom Damania, Dirk P. Dittmer
    PLoS Pathogens
  44. Alignment of EphA4 and ephrin-B2 expression patterns with developing modularity in the lateral cortex of the inferior colliculus.
    Authors: Sean M. Gay, Cooper A. Brett, Jeremiah P.C. Stinson, Mark L. Gabriele
    Journal of Comparative Neurology
  45. Anchoring and synaptic stability of PSD-95 is driven by ephrin-B3
    Authors: Martin Hruska, Nathan T. Henderson, Nan L. Xia, Sylvain J. Le Marchand, Matthew B. Dalva
    Nature Neuroscience
  46. Type I Diabetes Delays Perfusion and Engraftment of 3D Constructs by Impinging on Angiogenesis; Which can be Rescued by Hepatocyte Growth Factor Supplementation
    Authors: Wafa Altalhi, Rupal Hatkar, James B. Hoying, Yasaman Aghazadeh, Sara S. Nunes
    Cellular and Molecular Bioengineering

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Reviews for Human/Mouse/Rat Ephrin-B2 Antibody

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Human/Mouse/Rat Ephrin-B2 Antibody
By Anonymous on 03/16/2021
Application: WB
Reason for Rating: Works well. Unambiguous results!! Sample Tested: Cell culture supernatant,Cell Lysates Species: Human
Western Blot Human/Mouse/Rat Ephrin-B2 Antibody AF496
Human/Mouse/Rat Ephrin-B2 Antibody
By Anonymous on 12/16/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: E12.5 mouse embryo fixed in 4% PFA Species: Mouse

Dilution used - 1:200. The staining was done on an E12.5 transverse mouse section fixed overnight with 4% PFA.
Stained using standard IF technique.
Attached picture shows an artery and a vein and it is clearly seen that the artery seen is stained positive whereas the adjacent vein is negative for EphrinB2 (as it should be).

Immunocytochemistry/Immunofluorescence Human/Mouse/Rat Ephrin-B2 Antibody AF496