Human/Mouse/Rat CIB1 Antibody Summary
Gly2-Leu191
Accession # Q99828
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human, Mouse, and Rat CIB1 antibody by Western Blot. Detection of Human, Mouse, and Rat CIB1 antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/CIB1_AF7557_Western_Blot_12313.jpg)
Detection of Human, Mouse, and Rat CIB1 by Western Blot. Western blot shows lysates of human spleen tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat CIB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CIB1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CIB1
CIB1 (Calcium and Integrin-Binding protein 1; also calmyrin, KIP and CIBP) is a 22-23 kDa, Ca++-binding member of the CIB family of proteins. It is widely expressed, being found in neurons, platelets/megakaryocytes, skeletal muscle myocytes and lymphocytes. CIB1 is associated with the cytosolic side of the plasma membrane, and has multiple binding partners, including InsP3R, GPIIb/ alpha IIb, presenilin 2, and NBR1 plus FEZ. When CIB1 binds InsP3R, this ER-embedded receptor is both activated, and later desensitized to subsequent ligand binding. Relative to GPIIb, CIB1 interaction with the alpha IIb beta 3 integrin on platelets following thrombin exposure appears to inhibit integrin activation, thus providing a tight control on subsequent platelet binding to fibrinogen. Human CIB1 is 191 amino acids (aa) in length. It contains a utilized myristoylation site at Gly2, followed by two EF-hand domains (aa 103-183). CIB1 is suggested to act as a monomer. There is at least one potential isoform variant that shows a 40 aa insertion after Lys29. Full-length human CIB1 (aa 1-191) shares 94% aa sequence identity with mouse CIB1.
Product Datasheets
Citation for Human/Mouse/Rat CIB1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Expression of a TMC6-TMC8-CIB1 heterotrimeric complex in lymphocytes is regulated by each of the components
Authors: CJ Wu, X Li, CL Sommers, K Kurima, S Huh, G Bugos, L Dong, W Li, AJ Griffith, LE Samelson
J. Biol. Chem., 2020-09-11;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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