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Human/Mouse/Rat CCK-AR Antibody

Catalog #: AF2680
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2680
AF2680-SP
CCK-A R in Rat Pancreas.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat CCK-AR Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat CCK-A R.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Rat CCK-A R synthetic peptide
Lys256-Glu267
Accession # P30551
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCK-A R
Recombinant Mouse CCK-A R
Recombinant Rat CCK-A R
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CCK-A R antibody in Rat Pancreas by Immunohistochemistry (IHC-Fr). View Larger

CCK-A R in Rat Pancreas. CCK-A R was detected in perfusion fixed frozen sections of rat pancreas using 5 µg/mL Human/Mouse/Rat CCK-A R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2680) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCK-AR

The physiologic actions of the cholecystokinin (CCK) family of peptide hormones are mediated through two classes of G protein-coupled receptors, CCK-A R and CCK-B R. CCK-A R is a 428 amino acid protein with 7 putative transmembrane domains. This GPCR is involved in gallbladder contraction, insulin secretion, and has also been described in specific regions of the central nervous system. CCK-A R has been implicated in pancreatic tumorigenesis as well as the pathogenesis of eating disorders and drug addiction.

Long Name
Cholecystokinin Receptor, Type A
Entrez Gene IDs
886 (Human); 12425 (Mouse)
Alternate Names
CCK1R; CCK1-R; CCK-A R; CCK-A receptor; CCK-A; CCKAR; CCK-AR; CCKRA; cholecystokinin A receptor; cholecystokinin receptor type A; cholecystokinin type-A receptor; Cholecystokinin-1 receptor

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Citations for Human/Mouse/Rat CCK-AR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A Novel CCK Receptor GPR173 Mediates Potentiation of GABAergic Inhibition
    Authors: He L, Shi H, Zhang G et al.
    The Journal of Neuroscience
  2. In vitro analysis of the effects of cholecystokinin on rat brain stem motoneurons.
    Authors: Zheng Z, Lewis MW, Travagli RA
    Am. J. Physiol. Gastrointest. Liver Physiol., 2004-12-09;288(5):G1066-73.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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