Human/Mouse/Rat Caveolin-1 Antibody

Name: Human/Mouse/Rat Caveolin-1 Antibody
Brand: R&D Systems
SKU: MAB5736
Rating: 5 (1 reviews)

Catalog #: MAB5736
9 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5736
MAB5736-SP

Detection of Human Caveolin‑1 by Western Blot.

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Product Details

Citations (9)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Caveolin-1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat Caveolin-1 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 7C8

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Puified rat adipocyte low density microsomes
Accession # P41350

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Caveolin‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells and HMVEC human microvascular endothelial cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Caveolin-1 Monoclonal Antibody (Catalog # MAB5736) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caveolin-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry

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Caveolin‑1 in A431 Human Cell Line. Caveolin-1 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Caveolin-1 Monoclonal Antibody (Catalog # MAB5736) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caveolin-1

Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae, the cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and regulation of lipid rafts. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (aa 105‑125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Within these cytoplasmic regions, human Caveolin-1 shares 95% aa sequence identity with mouse and rat Caveolin-1. Alternate splicing in human, mouse and rat generates an isoform with a deletion of the N-terminal 31 residues.

Entrez Gene IDs

857 (Human); 12389 (Mouse); 25404 (Rat)

Alternate Names

CAV; CAV1; caveolae protein, 22kD; caveolin 1, caveolae protein, 22kDa; Caveolin1; Caveolin-1; cell growth-inhibiting protein 32; MSTP085; VIP21

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Caveolin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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PKM2 promotes pulmonary fibrosis by stabilizing TGF-beta1 receptor I and enhancing TGF-beta1 signaling
Authors: S Gao, X Li, Q Jiang, Q Liang, F Zhang, S Li, R Zhang, J Luan, J Zhu, X Gu, T Xiao, H Huang, S Chen, W Ning, G Yang, C Yang, H Zhou
Science Advances, 2022-09-21;8(38):eabo0987.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Fatty acid mobilization from adipose tissue is mediated by CD36 post-translational modifications and intracellular trafficking
Authors: AC Daquinag, Z Gao, C Fussell, L Immaraj, R Pasqualini, W Arap, AM Akimzhanov, M Febbraio, MG Kolonin
JCI Insight, 2021-09-08;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Proteomic Analyses of Fibroblast- and Serum-Derived Exosomes Identify QSOX1 as a Marker for Non-invasive Detection of Colorectal Cancer
Authors: N Ganig, F Baenke, ML Thepkayson, K Lin, VS Rao, FC Wong, H Polster, M Schneider, D Helm, M Pecqueux, AM Seifert, L Seifert, J Weitz, NN Rahbari, C Kahlert
Cancers, 2021-03-17;13(6):.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot
SMN protein promotes membrane compartmentalization of ribosomal protein S6 transcript in human fibroblasts
Authors: F Gabanella, A Onori, M Ralli, A Greco, C Passananti, MG Di Certo
Sci Rep, 2020-11-04;10(1):19000.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
Hyperthermia and associated changes in membrane fluidity potentiate P2X7 activation to promote tumor cell death.
Authors: de Andrade Mello P, Bian S, Savio L, Zhang H, Zhang J, Junger W, Wink M, Lenz G, Buffon A, Wu Y, Robson S
Oncotarget, 2017-06-21;8(40):67254-67268.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation
A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells.
Authors: Liu C, Liu T, Yu X, Gu Y
Acta Pharm Sin B, 2017-04-01;7(3):361-372.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Enterohemorrhagic Escherichia coli hemolysin employs outer membrane vesicles to target mitochondria and cause endothelial and epithelial apoptosis.
Authors: Bielaszewska M, Ruter C, Kunsmann L, Greune L, Bauwens A, Zhang W, Kuczius T, Kim K, Mellmann A, Schmidt M, Karch H
PLoS Pathog, 2013-12-12;9(12):e1003797.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Regulation of amino acid/carnitine transporter B 0,+ (ATB 0,+) in astrocytes by protein kinase C: independent effects on raft and non-raft transporter subpopulations.
Authors: Samluk L, Czeredys M, Nalecz K
J Neurochem, 2010-10-26;115(6):1386-97.
Species: Rat
Sample Types: Protein, Whole Cells
Applications: ICC, Western Blot
Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signaling.
Authors: Souto R, Vallega G, Wharton J, Vinten J, Tranum-Jensen J, Pilch P
J Biol Chem, 2003-03-11;278(20):18321-9.
Species: Rat
Sample Types: Whole Cells
Applications: Immunoprecipitation, Western Blot