Human/Mouse/Rat ARC Antibody

Catalog #: AF636
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF636
AF636-SP

Detection of Human/Mouse/Rat ARC by Western Blot.

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Human/Mouse/Rat ARC Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat ARC in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

KLH-coupled human ARC synthetic peptide
CEPEAEAEPEPELE PEPDPEP

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human brain (hippocampus), heart and skeletal muscle, mouse skeletal muscle, and rat brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat ARC by Western Blot. Western blot shows lysates of rat and mouse skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat ARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF636) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human ARC Western Blot Standard (Catalog # WBC009) (1 ng) was included. A specific band for ARC was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ARC

ARC [Apoptosis Repressor with CARD (Caspase-recruitment Domain)] is a 29 kDa cytosolic protein containing an N-terminal CARD joined to a C-terminal region rich in proline and glutamic acid. The expression of human ARC is primarily restricted to skeletal muscle and cardiac tissue. ARC inhibits apoptosis induced by Caspase-8.

Long Name

Apoptosis Repressor with Caspase Recruitment Domain

Entrez Gene IDs

8996 (Human); 11838 (Mouse)

Alternate Names

Apoptosis repressor with CARD; ARC; ARCFLJ35304; CARD2; Muscle-enriched cytoplasmic protein; MYC; MYP; NOL3; NOP30; NOPnucleolar protein 3; nucleolar protein 3 (apoptosis repressor with CARD domain); Nucleolar protein of 30 kDa

Product Datasheets

Product Datasheet

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SDS

Related Research Areas

Citation for Human/Mouse/Rat ARC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1