Human/Mouse Proprotein Convertase 2/PCSK2 Antibody
Human/Mouse Proprotein Convertase 2/PCSK2 Antibody Summary
Gly110-Asn638
Accession # P16519
Applications
PCSK2 (Catalog # 6018-SE), see our available Western blot detection antibodies.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Proprotein Convertase 2/PCSK2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, beta TC-6 mouse beta cell insulinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Proprotein Convertase 2/PCSK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6018) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Proprotein Convertase 2/PCSK2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Proprotein Convertase 2/PCSK2 in HepG2 Human Cell Line. Proprotein Convertase 2/PCSK2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human/Mouse Proprotein Convertase 2/PCSK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue).This application has not been tested in mouse samples. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Proprotein Convertase 2/PCSK2
Proprotein Convertase 2 (PCSK2) is a subtilisin-like serine peptidase that processes proteins into biologically active products in the secretory pathway. Like other furin/kexin family members, PCSK2 cleaves its substrates primarily after sites of paired basic amino acid residues (1). Autoactivation of PCSK2 occurs in a post‑Golgi compartment of the secretory system (2). Interaction with the secretory protein 7B2 is required for proper activation of PCSK2 (3). The N-terminal domain of 7B2 stabilizes active PCSK2, and a C-terminal fragment of 7B2 can inhibit PCSK2 activity (4). rhPCSK2 was coexpressed with 7B2 to facilitate activation of the enzyme. PCSK2 is a major proteolytic processing enzyme of the regulated secretory pathway of the neuroendocrine system, where it generates a number of hormones and neuropeptides (5). Products of PCSK2 processing include enkephalins, insulin, somatostatin, dynorphin, and LHRH. Within the region used as an immunogen, human PCSK2 shares 97% aa identity with mouse PCSK2.
- Zhou, A. et al. (1999) J. Biol. Chem. 274:20745.
- Lamango, N.S. et al. (1999) Arch. Biochem. Biophys. 362:275.
- Zhu, X. and I. Lindberg (1995) J. Cell Biol. 129:1641.
- Lamango, N. et al. (1996) Arch. Biochem. Biophys. 330:238.
- Rouillé, Y. et al. (1995) Front. Neuroendocrinol. 16:322.
Product Datasheets
Citations for Human/Mouse Proprotein Convertase 2/PCSK2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Posttranslational processing of FGF23 in osteocytes during the osteoblast to osteocyte transition
Authors: Hiroyuki Yamamoto, Bruno Ramos-Molina, Adam N. Lick, Matthew Prideaux, Valeria Albornoz, Lynda Bonewald et al.
Bone
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Tbr1 instructs laminar patterning of retinal ganglion cell dendrites
Authors: J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes
Nat. Neurosci., 2018-04-09;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
FAQs
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