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Human/Mouse PRDM16/MEL1 Antibody

Catalog #: AF6295
20 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6295
AF6295-SP
Detection of Human PRDM16 by Western Blot.
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Product Details
Citations (20)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse PRDM16/MEL1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse and human PRDM16 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse PRDM16
Lys537-Glu688
Accession # A2A935.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PRDM16 antibody by Western Blot. View Larger

Detection of Human PRDM16 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRDM16 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry PRDM16 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

PRDM16 in Mouse Embryo. PRDM16 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (orange, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to the trigeminal ganglion. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRDM16/MEL1

PRDM16 (PR [PRDI-BF1 and RIZ] domain containing protein 16; also MEL-1) is a 170 kDa member of the PR Domain family of proteins. It is a transcriptional regulator expressed in the embryo, and is reported to participate in the maintenance of both neuronal and hematopoietic progenitor stem cells populations, and to preferentially promote the development of brown fat from adipomyocyte precursors. The generation of brown fat is likely due to suppression of muscle-specific factors. Mouse PRDM16 is 1275 amino acids (aa) in length. It contains one SET domain (aa 85-208) followed by ten C2H2 type Zn finger motifs (aa 230-1030). There are multiple potential isoform variants that likely vary from 150-170 kDa in size. One isoform shows a deletion of aa 1232-1250, a second isoform shows a three aa substitution for aa 1174-1275, and a third isoform possesses an alternative start site at Met21, coupled to a deletion of aa 1196-1133. Over aa 537-688, mouse PRDM16 shares 81% and 95% aa identity with human and rat PRDM16, respectively.

Long Name
PR Domain Containing 16
Entrez Gene IDs
63976 (Human); 70673 (Mouse)
Alternate Names
MDS1/EVI1-like gene 1; MEL1; MEL1KIAA1675PFM13MGC166915; PFM13; PR domain containing 16; PR domain zinc finger protein 16; PR domain-containing protein 16; PRDM16; Transcription factor MEL1

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Citations for Human/Mouse PRDM16/MEL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. PRDM16 co-operates with LHX2 to shape the human brain
    Authors: Suresh, V;Bhattacharya, B;Tshuva, RY;Danan Gotthold, M;Olender, T;Bose, M;Pradhan, SJ;Ben Zeev, B;Smith, RS;Tole, S;Galande, S;Harwell, C;Baizabal, JM;Reiner, O;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  2. Post-translational control of beige fat biogenesis by PRDM16 stabilization
    Authors: Q Wang, H Li, K Tajima, ARP Verkerke, ZH Taxin, Z Hou, JB Cole, F Li, J Wong, I Abe, RN Pradhan, T Yamamuro, T Yoneshiro, JN Hirschhorn, S Kajimura
    Nature, 2022-08-17;609(7925):151-158.
    Species: Mouse
    Sample Types:
  3. Population genomics reveals that natural variation in PRDM16 contributes to cold tolerance in domestic cattle
    Authors: Chun-Long Yan, 延边大学农学院, 吉林 延吉133000, College of Agriculture, Yanbian University, Yanji, Jilin 133000, China, Jun Lin, Yuan-Yuan Huang, Qing-Shan Gao et al.
    Zoological Research
  4. Defective brown adipose tissue thermogenesis and impaired glucose metabolism in mice lacking Letmd1
    Authors: KM Choi, JH Kim, X Kong, M Isik, J Zhang, HW Lim, JC Yoon
    Cell Reports, 2021-12-14;37(11):110104.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Early postnatal interactions between beige adipocytes and sympathetic neurites regulate innervation of subcutaneous fat
    Authors: Jingyi Chi, Zeran Lin, William Barr, Audrey Crane, Xiphias Ge Zhu, Paul Cohen
    eLife
  6. PRDM16 orchestrates angiogenesis via neural differentiation in the developing brain
    Authors: L Su, X Lei, H Ma, C Feng, J Jiang, J Jiao
    Cell Death Differ., 2020-02-03;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  7. Gain of Metabolic Benefit with Ablation of miR-149-3p from Subcutaneous Adipose Tissue in Diet-Induced Obese Mice
    Authors: Shasha Zheng, Shanjun Guo, Gongrui Sun, Yanteng Shi, Zhe Wei, Yuhang Tang et al.
    Molecular Therapy - Nucleic Acids
  8. Novel discovery of Averrhoa bilimbi ethanolic leaf extract in the stimulation of brown fat differentiation program in combating diet-induced obesity
    Authors: WK Lau, NAA Noruddin, AH Ariffin, MZ Mahmud, MHM Noor, A Amanah, MF Hamzah, Z Zafarina
    BMC Complement Altern Med, 2019-09-05;19(1):243.
    Species: Human
    Sample Types: Whole Cells
    Applications: Screening
  9. Cbx4 Sumoylates Prdm16 to Regulate Adipose Tissue Thermogenesis
    Authors: Q Chen, L Huang, D Pan, LJ Zhu, YX Wang
    Cell Rep, 2018-03-13;22(11):2860-2872.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. The Transcription Factor Prdm16 Marks a Single Retinal Ganglion Cell Subtype in the Mouse Retina
    Authors: S Groman-Lup, J Adewumi, KU Park, JA Brzezinski
    Invest. Ophthalmol. Vis. Sci., 2017-10-01;58(12):5421-5433.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Transcription factor Hlx controls a systematic switch from white to brown fat through Prdm16-mediated co-activation
    Authors: L Huang, D Pan, Q Chen, LJ Zhu, J Ou, M Wabitsch, YX Wang
    Nat Commun, 2017-07-12;8(1):68.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  12. Loss of ADAMTS5 enhances brown adipose tissue mass and promotes browning of white adipose tissue via CREB signaling
    Authors: D Bauters, M Cobbaut, L Geys, J Van Lint, B Hemmeryckx, HR Lijnen
    Mol Metab, 2017-05-10;6(7):715-724.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Lysine-specific demethylase 1 promotes brown adipose tissue thermogenesis via repressing glucocorticoid activation
    Authors: Xing Zeng, Mark P. Jedrychowski, Yi Chen, Sara Serag, Gareth G. Lavery, Steve P. Gygi et al.
    Genes & Development
  14. Fasting induces a subcutaneous-to-visceral fat switch mediated by microRNA-149-3p and suppression of PRDM16
    Authors: Hanying Ding
    Nat Commun, 2016-05-31;7(0):11533.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
    Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C
    Nat Cell Biol, 2014-12-08;17(1):57-67.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. MicroRNA-378 controls classical brown fat expansion to counteract obesity.
    Authors: Pan, Dongning, Mao, Chunxiao, Quattrochi, Brian, Friedline, Randall, Zhu, Lihua J, Jung, Dae Youn, Kim, Jason K, Lewis, Brian, Wang, Yong-Xu
    Nat Commun, 2014-08-22;5(0):4725.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. KSRP ablation enhances brown fat gene program in white adipose tissue through reduced miR-150 expression.
    Authors: Chou C, Lin Y, Wang H, Zhu X, Giovarelli M, Briata P, Gherzi R, Garvey W, Chen C
    Diabetes, 2014-04-10;63(9):2949-61.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. MyomiR-133 regulates brown fat differentiation through Prdm16.
    Authors: Trajkovski, Mirko, Ahmed, Kashan, Esau, Christin, Stoffel, Markus
    Nat Cell Biol, 2012-12-01;14(12):1330-5.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Brown remodeling of white adipose tissue by SirT1-dependent deacetylation of Ppargamma.
    Authors: Qiang L, Wang L, Kon N, Zhao W, Lee S, Zhang Y, Rosenbaum M, Zhao Y, Gu W, Farmer S, Accili D
    Cell, 2012-08-03;150(3):620-32.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Prdm16 and Vcam1 regulate the postnatal disappearance of embryonic radial glia and the ending of cortical neurogenesis
    Authors: J Li, MI Godoy, AJ Zhang, G Diamante, IS Ahn, A Cebrian-Si, A Alvarez-Bu, X Yang, BG Novitch, Y Zhang
    bioRxiv : the preprint server for biology, 2023-02-14;0(0):.

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Reviews for Human/Mouse PRDM16/MEL1 Antibody

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Human/Mouse PRDM16/MEL1 Antibody
By Anonymous on 03/08/2016
Application: WB Sample Tested: Adipose tissue,Brown adipose tissue Species: Mouse

Samples were nuclear extracts from brown adipose and subcutaneous adipose tissue (20ug protein loaded). Primary antibody (1:1,000) was detected with HRP-anti sheep (1:10,000) then amplified with Licor streptavidin 800 (1:10,000). Near infrared fluorescence gave much better signal-to-noise than chemiluminescence.

Western Blot Human/Mouse PRDM16/MEL1 Antibody AF6295