Home / PDGF R beta / Human/Mouse Phospho-PDGF R beta (Y751) Antibody

Human/Mouse Phospho-PDGF R beta (Y751) Antibody

Catalog #: MAB9027
2 Citations Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9027
MAB9027-SP
Detection of Human and Mouse Phospho-PDGF R beta (Y751) by Western Blot.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Phospho-PDGF R beta (Y751) Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse PDGF R beta when phosphorylated at Y751 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1210B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing the human PDGF R beta (Y751) site
Accession # P09619
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Phospho-PDGF R beta (Y751) antibody by Western Blot. View Larger

Detection of Human and Mouse Phospho-PDGF R beta (Y751) by Western Blot. Western blot shows lysates of CCD-1070Sk human foreskin fibroblast cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse Phospho-PDGF R beta (Y751) Monoclonal Antibody (Catalog # MAB9027) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-PDGF R beta (Y751) antibody in BJ Human Cell Line by Immunocytochemistry (ICC). View Larger

Phospho-PDGF R beta (Y751) in BJ Human Cell Line. PDGF R beta phosphorylated at Y751 was detected in immersion fixed BJ human skin fibroblast cell line stimulated (top panel), and unstimulated (bottom panel), with Recombinant Human PDGF-BB (Catalog # 220-BB) using Rabbit Anti-Human/Mouse Phospho-PDGF R beta (Y751) Monoclonal Antibody (Catalog # MAB9027) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Mouse Phospho-PDGF R beta (Y751) antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Phospho-PDGF R beta (Y751) by Simple WesternTM. Simple Western lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for PDGF R beta at approximately 240 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse Phospho-PDGF R beta (Y751) Monoclonal Antibody (Catalog # MAB9027). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF‑B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin‑like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.

References
  1. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
Long Name
Platelet-derived Growth Factor Receptor beta
Entrez Gene IDs
5159 (Human); 18596 (Mouse); 24629 (Rat)
Alternate Names
beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide

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Citations for Human/Mouse Phospho-PDGF R beta (Y751) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Retinal inflammation in murine models of type 1 and type 2 diabetes with diabetic retinopathy
    Authors: Subramanian Dharmarajan, Casandra Carrillo, Zhonghua Qi, Jonathan M. Wilson, Anthony J. Baucum, Christine M. Sorenson et al.
    Diabetologia
  2. Assembly of fibronectin fibrils selectively attenuates platelet-derived growth factor-induced intracellular calcium release in fibroblasts
    Authors: CS Farrar, DC Hocking
    J. Biol. Chem., 2018-10-15;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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