Human/Mouse PGPEP-1 Antibody

Catalog #: AF6278 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6278
AF6278-SP

Detection of Human and Mouse PGPEP‑1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse PGPEP-1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human PGPEP-1 in direct ELISAs and, human and mouse PGPEP-1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PGPEP-1
Met1-His209
Accession # Q9NXJ5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse PGPEP‑1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, JAR human choriocarcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PGPEP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6278) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PGPEP-1 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGPEP-1

Pyroglutamyl peptidase-1 (PGPEP-1) is an omega peptidase which removes pyroglutamyl residues from the amino termini of peptides and proteins (1). It is a cytosolic cysteine peptidase that is expressed in most cell types (2). The enzyme requires a thiol‑reducing agent for activity (3). PGPEP-1 is potentially involved in the inactivation of biologically active peptides that possess an amino terminal pyroglutamyl group (3). Examples of such peptides include neurotensin, luteinizing hormone releasing hormone, and thyrotropin-releasing hormone. Human and mouse PGPEP-1 share 95% aa sequence identity.

References

Kilbane Z. et al. (2007) Mol. Cell. Biochem. 297:189.
Cummins P.M. and B. O'Connor (1998) Biochim. Biophys. Acta. 1429:1.
Dando P.M. et al. (2003) Protein Express. Purif. 28:111.

Long Name

Pyroglutamyl Aminopeptidase I

Entrez Gene IDs

54858 (Human)

Alternate Names

EC 3.4.19.3; PAP-I; Pcp; PGPEP1; PGPEP-1; PGPIFLJ20208; PGP-IPAP-I; PGPMGC10812; Pyroglutamyl aminopeptidase I; pyroglutamyl-peptidase 1; pyroglutamyl-peptidase I5-oxoprolyl-peptidase; Pyrrolidone-carboxylate peptidase