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Human/Mouse Osteocrin Antibody

Catalog #: MAB2620
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2620
MAB2620-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Osteocrin Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Osteocrin in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 311417
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Osteocrin
Val28-Gly130
Accession # P61364
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Osteocrin
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human or Recombinant Mouse Osteocrin, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteocrin

Mouse Osteocrin, also known as Musclin, is a secreted protein that is primarily expressed in bone and muscle. It is synthesized as an 11 kDa proprotein that undergoes proteolytic processing to generate a mature 50 amino acid (5 kDa) C-terminal active peptide. It was found to modulate osteoblast differentation and to regulate glucose metabolism in muscles. Mouse Osteocrin proprotein shares 77% and 78% amino acid sequence identity with the rat and human protein, respectively.

Entrez Gene IDs
344901 (Human); 239790 (Mouse)
Alternate Names
Musclin; Osteocrin; OSTN

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Citation for Human/Mouse Osteocrin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Mechanical load regulates bone growth via periosteal Osteocrin
    Authors: H Watanabe-T, H Ochi, A Chiba, A Matsuo, Y Kanai, S Fukuhara, N Ito, K Sako, T Miyazaki, K Tainaka, I Harada, S Sato, Y Sawada, N Minamino, S Takeda, HR Ueda, A Yasoda, N Mochizuki
    Cell Reports, 2021-07-13;36(2):109380.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Capture

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