Human/Mouse Orexin A/Hypocretin-1 Biotinylated Antibody

Detection of Orexin A/Hypocretin‑1 in Human Hypothalamus. Orexin A/Hypocretin‑1 was detected in immersion fixed paraffin-embedded sections of Human Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin‑1 Biotinylated Monoclonal Antibody (Catalog # BAM763) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Orexin A/Hypocretin‑1 in Mouse Hypothalamus. Orexin A/Hypocretin‑1 was detected in perfusion fixed frozen sections of Mouse Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin‑1 Biotinylated Monoclonal Antibody (Catalog # BAM763) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.