Human/Mouse NLRP3/NALP3 APC-conjugated Antibody

Name: Human/Mouse NLRP3/NALP3 APC-conjugated Antibody
Brand: R&D Systems
SKU: IC7578A
Rating: 5 (1 reviews)

Catalog #: IC7578A
4 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
IC7578A

Detection of NLRP3/NALP3 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

2 Images

All NLRP3/NALP3 Antibodies

Product Details

Citations (4)

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse NLRP3/NALP3 APC-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse NLRP3/NALP3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human NLRP3/NALP3 is observed.

Source

Monoclonal Rat IgG_2A Clone # 768319

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse NLRP3/NALP3
Met1-Arg153
Accession # Q8R4B8

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of NLRP3/NALP3 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

View Larger

Detection of NLRP3/NALP3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse NLRP3/NALP3 APC-conjugated Monoclonal Antibody (Catalog # IC7578A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

Detection of NLRP3/NALP3 antibody in Human Blood Monocytes antibody by Flow Cytometry.

View Larger

Detection of NLRP3/NALP3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human/Mouse NLRP3/NALP3 APC-conjugated Monoclonal Antibody (Catalog # IC7578A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NLRP3/NALP3

NLRP3, also known as NALP3, CIAS1, PYPAF or Cryopyrin, is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Mouse NLRP3 contains an N-terminal Pyrin domain (aa 1-91) followed by a Nacht region (aa 216-532), and seven LRRs (aa 739-988). Within the sequence used as an immunogen, mouse NLRP3 shares 78% and 93% aa identity with human and rat NLRP3, respectively. Alternate splicing of mouse NLRP3 generates additional isoforms that lack either LRR2 and 3, LRR 6 and 7, or LRR4-9.

Long Name

NLR Family, Pyrin Domain Containing 2

Entrez Gene IDs

114548 (Human); 216799 (Mouse); 287362 (Rat)

Alternate Names

AGTAVPRL; AII; AII/AVP; Angiotensin/vasopressin receptor AII/AVP-like; AVP; C1orf7; Caterpiller protein 1.1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cold autoinflammatory syndrome 1; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Leucine-rich repeat-, and PYD-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; NLR family, pyrin domain containing 3; NLRP3; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3; PYPAF1; PYRIN-containing APAF1-like protein 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse NLRP3/NALP3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

Recovery of Bone Marrow Function in VEXAS Syndrome-potential Role for Romiplostim
Authors: Adam Al-Hakim, Alyssa Cull, Joanna Topping, Fatima Nadat, Joanna Milek, Razan Alhefzi et al.
HemaSphere
MiR-223-3p attenuates radiation-induced inflammatory response and inhibits the activation of NLRP3 inflammasome in macrophages
Authors: Zhang, M;Lan, H;Peng, S;Zhou, W;Wang, X;Jiang, M;Hong, J;Zhang, Q;
International immunopharmacology
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Ablation of NLRP3 inflammasome rewires MDSC function and promotes tumor regression
Authors: Iosif Papafragkos, Maria Grigoriou, Louis Boon, Andreas Kloetgen, Aikaterini Hatzioannou, Panayotis Verginis
Frontiers in Immunology
NLRP3 inflammasome in fibroblasts links tissue damage with inflammation in breast cancer progression and metastasis
Authors: N Ershaid, Y Sharon, H Doron, Y Raz, O Shani, N Cohen, L Monteran, L Leider-Tre, A Ben-Shmuel, M Yassin, M Gerlic, A Ben-Baruch, M Pasmanik-C, R Apte, N Erez
Nat Commun, 2019-09-26;10(1):4375.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry