Human/Mouse NLRP3/NALP3 Alexa Fluor® 750-conjugated Antibody Summary
Met1-Arg153
Accession # Q8R4B8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse NLRP3/NALP3 by Flow Cytometry The cell-specific expression pattern of NLRP3 in the fibrotic liver of BDL-treated mice. (A) Mice were anesthetized and sacrificed at 2 weeks after sham or BDL operation. NLRP3 positive cells of the mouse liver non-parenchymal cells were analyzed for T cells, endothelial cells, dendritic cells, and macrophages-specific markers (CD3e, CD31, CD11c, and F4/80, respectively). Mice were lethally irradiated and received whole bone marrow cell transplants from EGFP transgenic mice, followed by sham or BDL operation. At 2 weeks after sham or BDL operation, the proportion of NLRP3+ cells in BMMs (F4/80+ EGFP+) was analyzed by FACS. (B) Representative images of immunofluorescence analysis by confocal microscopy to track the expression of NLRP3 (red) in macrophages (F4/80+, pink) of bone marrow origin (EGFP+, green) in mouse liver tissue. Data are presented as the mean ± SEM. n = 6 per group. Student's t-test was used in panel (A). *p < 0.05 vs. sham group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32695095), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse NLRP3/NALP3 by Flow Cytometry The cell-specific expression pattern of NLRP3 in the fibrotic liver of BDL-treated mice. (A) Mice were anesthetized and sacrificed at 2 weeks after sham or BDL operation. NLRP3 positive cells of the mouse liver non-parenchymal cells were analyzed for T cells, endothelial cells, dendritic cells, and macrophages-specific markers (CD3e, CD31, CD11c, and F4/80, respectively). Mice were lethally irradiated and received whole bone marrow cell transplants from EGFP transgenic mice, followed by sham or BDL operation. At 2 weeks after sham or BDL operation, the proportion of NLRP3+ cells in BMMs (F4/80+ EGFP+) was analyzed by FACS. (B) Representative images of immunofluorescence analysis by confocal microscopy to track the expression of NLRP3 (red) in macrophages (F4/80+, pink) of bone marrow origin (EGFP+, green) in mouse liver tissue. Data are presented as the mean ± SEM. n = 6 per group. Student's t-test was used in panel (A). *p < 0.05 vs. sham group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32695095), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Preparation and Storage
Background: NLRP3/NALP3
NLRP3, also known as NALP3, CIAS1, PYPAF or Cryopyrin, is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Mouse NLRP3 contains an N-terminal Pyrin domain (aa 1-91) followed by a Nacht region (aa 216-532), and seven LRRs (aa 739-988). Within the sequence used as an immunogen, mouse NLRP3 shares 78% and 93% aa identity with human and rat NLRP3, respectively. Alternate splicing of mouse NLRP3 generates additional isoforms that lack either LRR2 and 3, LRR 6 and 7, or LRR4-9.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citations for Human/Mouse NLRP3/NALP3 Alexa Fluor® 750-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Single-Cell RNA Sequencing Identifies New Inflammation-Promoting Cell Subsets in Asian Patients With Chronic Periodontitis
Authors: Qian SJ, Huang QR, Chen RY et al.
Frontiers in immunology
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Macrophage Sphingosine 1-Phosphate Receptor 2 Blockade Attenuates Liver Inflammation and Fibrogenesis Triggered by NLRP3 Inflammasome
Authors: Lei Hou, Le Yang, Na Chang, Xinhao Zhao, Xuan Zhou, Chengbin Dong et al.
Frontiers in Immunology
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