Human/Mouse Lumican Antibody

Catalog #: MAB2846
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2846
MAB2846-SP

Detection of Human Lumican by Western Blot.

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All Lumican Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Lumican Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Lumican in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 358022

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Lumican
Gln19-Asn338
Accession # P51884

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Lumican by Western Blot. Western blot shows lysates of human colon tissue and human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Lumican Monoclonal Antibody (Catalog # MAB2846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Lumican at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lumican

Lumican is a secreted matrix protein that belongs to the small leucine-rich proteoglycan (SLRP) class II family. Mature Lumican has a negatively charged N-terminal domain that contains tyrosine sulfates, followed by twelve leucine-rich repeats, which are flanked by cysteine loops. Lumican is expressed widely in mammalian connective tissues, while corneal Lumican exists as a keratan sulfate proteoglycan in adult cartilage, Lumican exists primarily as a glycoprotein and lacks keratan sulfate. The amino acid sequence of human Lumican shares 88% amino acid sequence identity with that of mature mouse Lumican.

Entrez Gene IDs

4060 (Human); 17022 (Mouse)

Alternate Names

KSPG lumican; LDC; LUM; lumican proteoglycan; Lumican; SLRR2D; SLRR2DLDCKeratan sulfate proteoglycan lumican

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse Lumican Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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