Human/Mouse JunD Antibody

Catalog #: AF5526 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5526
AF5526-SP

Detection of Human and Mouse JunD by Western Blot.

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Human/Mouse JunD Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse JunD in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human JunD
Lys47-Gly156
Accession # P17535

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse JunD by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, NIH-3T3 mouse embryonic fibroblast cell line, and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse JunD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5526) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for JunD at approximately 40 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JunD

JunD is a member of the Jun family of nuclear transcription factors. It dimerizes with other Jun or FOS family members to form the AP1 transcription factor. Unlike c-Jun or JunB, JunD antagonizes Ras transformation and accumulates as cells enter quiescence. JunD has two isoforms full length at 347 amino acids (aa), the other 303 aa which initiates translation at aa 44. Over the sequence used as an immunogen, human JunD shares 80% aa identity with mouse and rat JunD. The activation of JunD appears to reduce the proliferation of breast cancer cells.

Long Name

JunD Proto-oncogene

Entrez Gene IDs

3727 (Human); 16478 (Mouse); 24518 (Rat)

Alternate Names

activator protein 1; AP-1; jun D proto-oncogene; JunD; JunD-FL isoform; transcription factor jun-D