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Human/Mouse Ghrelin Antibody

Catalog #: MAB8200
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8200
MAB8200-SP
Ghrelin in Mouse Stomach.
2 Images
Product Details
Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Ghrelin Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse and human Ghrelin/Obestatin prepropeptide in ELISA. Detects human Ghrelin peptides (acylated and de-acylated) in ELISA.
Source
Monoclonal Rat IgG2A Clone # 883622
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Obestatin/Ghrelin prepropeptide
Gly24-Lys117
Accession # Q9EQX0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Ghrelin antibody in Mouse Stomach by Immunohistochemistry (IHC-Fr). View Larger

Ghrelin in Mouse Stomach. Ghrelin was detected in immersion fixed frozen sections of mouse stomach using Rat Anti-Human/Mouse Ghrelin Monoclonal Antibody (Catalog # MAB8200) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining is shown at both 60x magnification (upper panel) and 20x magnification (lower panel). Staining was localized to cytoplasm and gastric endocrine cells, respectively. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Ghrelin antibody in Human Stomach by Immunohistochemistry (IHC-P). View Larger

Ghrelin in Human Stomach. Ghrelin was detected in immersion fixed paraffin-embedded sections of human stomach using Rat Anti-Human/Mouse Ghrelin Monoclonal Antibody (Catalog # MAB8200) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ghrelin

GHRL (Ghrelin/Obestatin Prepropeptide) is a 117 aa precursor peptide that is processed into two chains, Ghrelin (aa24-51) and Obestatin (aa76-98).  The 18 kDa prepropeptide is cleaved into proGhrelin, and then further processed into mature Ghrelin by prohormone convertases.  Mature Ghrelin is modified with an N-octanoyl group on serine 3 which is required for receptor binding.  Ghrelin and Obestatin are predominantly synthesized in the gastric mucosa.  Ghrelin plays a role in growth factor release and appetite suppression as well as many other functions in a variety of organs.  Obestatin is a putative hormone that has been suggested to have opposite effects on growth factor release and appetite as Ghrelin. 

Entrez Gene IDs
51738 (Human); 58991 (Mouse); 59301 (Rat)
Alternate Names
appetite-regulating hormone; Ghrelin; ghrelin/obestatin preprohormone; ghrelin/obestatin prepropeptide; GHRL; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related Peptide; MTLRPgrowth hormone secretagogue receptor ligand; obestatin; Protein M46

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Citations for Human/Mouse Ghrelin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. FOXO1 Is Present in Stomach Epithelium and Determines Gastric Cell Distribution
    Authors: McKimpson WM, Kuo T, Kitamoto T et al.
    Gastro Hep Advances
  2. Pancreatic Nonhormone Expressing Endocrine Cells in Children With Type 1 Diabetes
    Authors: Md Moin AS, Cory M, Ong A et al.
    J Endocr Soc
  3. Limited extent and consequences of pancreatic SARS-CoV-2 infection
    Authors: Verena van der Heide, Sonia Jangra, Phillip Cohen, Raveen Rathnasinghe, Sadaf Aslam, Teresa Aydillo et al.
    Cell Reports
  4. Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans
    Authors: XX Yu, WL Qiu, L Yang, YC Wang, MY He, D Wang, Y Zhang, LC Li, J Zhang, Y Wang, CR Xu
    Cell Research, 2021-03-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Bariatric surgery induces a new gastric mucosa phenotype with increased functional glucagon-like peptide-1 expressing cells
    Authors: L Ribeiro-Pa, AC Jarry, JB Cavin, A Willemetz, J Le Beyec, A Sannier, S Benadda, AL Pelletier, M Hourseau, T Léger, B Morlet, A Couvelard, Y Anini, S Msika, JP Marmuse, S Ledoux, M Le Gall, A Bado
    Nature Communications, 2021-01-04;12(1):110.
    Species: Rat
    Sample Types: Whole Tissues
    Applications: IHC
  6. Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals
    Authors: T Yung, F Poon, M Liang, S Coquenlorg, EC McGaugh, CC Hui, MD Wilson, MC Nostro, TH Kim
    Nat Commun, 2019-10-11;10(1):4647.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  7. Neurogenin3 phosphorylation controls reprogramming efficiency of pancreatic ductal organoids into endocrine cells
    Authors: R Azzarelli, S Rulands, S Nestorowa, J Davies, S Campinoti, S Gillotin, P Bonfanti, B Göttgens, M Huch, B Simons, A Philpott
    Sci Rep, 2018-10-18;8(1):15374.
    Species: Mouse
    Sample Types: Organoids
    Applications: IHC
  8. Neuropeptide Y expression marks partially differentiated ? cells in mice and humans
    Authors: P Rodnoi, M Rajkumar, ASM Moin, SK Georgia, AE Butler, S Dhawan
    JCI Insight, 2017-06-15;2(12):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Characterization of Non-hormone Expressing Endocrine Cells in Fetal and Infant Human Pancreas.
    Authors: Moin ASM, Montemurro C, Zeng K et al.
    Front Endocrinol (Lausanne)

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