Human/Mouse GATA-3 Antibody

Catalog # Availability Size / Price Qty
MAB6330
MAB6330-SP
Detection of GATA-3 in Human Breast Cancer via Multiplex Immunofluorescence staining on COMET™​
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Product Details
Citations (16)
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Reviews (1)

Human/Mouse GATA-3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse GATA-3 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 634913
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Dual RNAscope ISH-IHC
5-25 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below
Multiplex Immunofluorescence
20 µg/mL
Paraffin embedded tissue sections of Human Breast Cancer

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Multiplex Immunofluorescence View Larger

Detection of GATA-3 in Human Breast Cancer via Multiplex Immunofluorescence staining on COMET™​ GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer using Mouse Anti-Human GATA‑3 Monoclonal Antibody (MAB6330) at 20 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus with dim cytoplasmic background signal. Protocol available in COMET™ Panel Builder.

Western Blot Detection of Human and Mouse GATA-3 antibody by Western Blot. View Larger

Detection of Human and Mouse GATA‑3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Moue Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for full length (FL) GATA-3 at approximately 52 kDa and the splice form (SF) found in MCF-7 cells at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry GATA-3 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

GATA‑3 in MCF‑7 Human Cell Line. GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry GATA-3 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

GATA‑3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human GATA-3 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human GATA‑3 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. Specific bands were detected for GATA‑3 at approximately 53 (splice variant) and 61 kDa (full length) as indicated, using 10 µg/mL of Mouse Anti-Human/Mouse GATA‑3 Monoclonal Antibody (Catalog # MAB6330). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Dual RNAscope ISH-IHC Compatible GATA-3 antibody in Human Breast Cancer Tissue Using Dual RNAscope® ISH and IHC. View Larger

GATA-3 in Human Breast Cancer Tissue Using Dual RNAscope® ISH and IHC. GATA-3 mRNA (red) and protein (green) was detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer tissue probed with ACD RNAScope® Probe (Catalog # 403551) followed by immunohistochemistry using R&D Systems Mouse Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog# MAB6330) at 5 μg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (VC001). Tissue was stained using ACD RNAscope® 2.5 HD Duplex Detection Reagents (Catalog # 322500).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-3

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.

Entrez Gene IDs
2625 (Human); 14462 (Mouse)
Alternate Names
131320; GATA binding protein 3; GATA3; GATA-3; GATA-binding factor 3; GATA-binding protein 3; HDRMGC5199; MGC2346; MGC5445; trans-acting T-cell-specific transcription factor GATA-3

Product Datasheets

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Citations for Human/Mouse GATA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Annexin A1 is a cell-intrinsic metalloregulator of zinc in human ILC2s
    Authors: Irie, M;Kabata, H;Sasahara, K;Kurihara, M;Shirasaki, Y;Kamatani, T;Baba, R;Matsusaka, M;Koga, S;Masaki, K;Miyata, J;Araki, Y;Kikawada, T;Kabe, Y;Suematsu, M;Yamagishi, M;Uemura, S;Moro, K;Fukunaga, K;
    Cell reports
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Endothelial-to-osteoblast transition in normal mouse bone development
    Authors: Song-Chang Lin, Guoyu Yu, Yu-Chen Lee, Jian H. Song, Xingzhi Song, Jianhua Zhang et al.
    iScience
  3. Analysis of rheumatoid- vs psoriatic arthritis synovial fluid reveals differential macrophage (CCR2) and T helper subsets (STAT3/4 and FOXP3) activation
    Authors: F Caso, A Saviano, M Tasso, F Raucci, N Marigliano, S Passavanti, P Frallonard, R Ramonda, V Brancaleon, M Bucci, R Scarpa, L Costa, F Maione
    Autoimmunity reviews, 2022-10-01;21(12):103207.
    Species: Human
    Sample Types: Synovial Fluid
    Applications: Western Blot
  4. Trophectoderm differentiation to invasive syncytiotrophoblast is promoted by endometrial epithelial cells during human embryo implantation
    Authors: Peter T Ruane, Terence Garner, Lydia Parsons, Phoebe A Babbington, Ivan Wangsaputra, Susan J Kimber et al.
    Human Reproduction
  5. Predictive role of endometrial T-bet/GATA3 ratio during mid-luteal phase for live birth in patients undergoing in vitro fertilization: A retrospective observational study
    Authors: Y Li, S Yu, C Huang, L Diao, C Chen, W Liu, R Lian, M Mo, C Du, F Liu, Y Zeng
    Journal of reproductive immunology, 2021-12-16;149(0):103465.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Generation of human blastocyst-like structures from pluripotent stem cells
    Authors: Y Fan, Z Min, S Alsolami, Z Ma, E Zhang, W Chen, K Zhong, W Pei, X Kang, P Zhang, Y Wang, Y Zhang, L Zhan, H Zhu, C An, R Li, J Qiao, T Tan, M Li, Y Yu
    Cell Discovery, 2021-09-07;7(1):81.
    Species: Human
    Sample Types: Spheroids
    Applications: IHC
  7. Transplantable human motor networks as a neuron-directed strategy for spinal cord injury
    Authors: Zachary T. Olmsted, Cinzia Stigliano, Annalisa Scimemi, Tatiana Wolfe, Jose Cibelli, Philip J. Horner et al.
    iScience
  8. Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer
    Authors: Naphannop Sereesongsaeng, Sara H. McDowell, James F. Burrows, Christopher J. Scott, Roberta E. Burden
    Breast Cancer Research
  9. Protein O-GlcNAcylation Promotes Trophoblast Differentiation at Implantation
    Authors: PT Ruane, CMJ Tan, DJ Adlam, SJ Kimber, DR Brison, JD Aplin, M Westwood
    Cells, 2020-10-06;9(10):.
    Species: Mouse
    Sample Types: Embryo, Whole Cells
    Applications: ICC, IHC
  10. IL-13 is produced by tumor cells in Breast Implant Associated Anaplastic Large Cell Lymphoma: Implications for pathogenesis
    Authors: ME Kadin, J Morgan, H Xu, AL Epstein, D Sieber, BA Hubbard, WP Adams, CE Bacchi, JCS Goes, MW Clemens, L Jeffrey Me, RN Miranda
    Hum. Pathol., 2018-04-22;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Increased TREM-2 expression on the subsets of CD11c(+) cells in the lungs and lymph nodes during allergic airway inflammation
    Authors: SC Hall, DK Agrawal
    Sci Rep, 2017-09-19;7(1):11853.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. ZNF503/Zpo2 drives aggressive breast cancer progression by down-regulation of GATA3 expression
    Authors: P Shahi, CY Wang, DA Lawson, EM Slorach, A Lu, Y Yu, MD Lai, H Gonzalez V, Z Werb
    Proc. Natl. Acad. Sci. U.S.A, 2017-03-03;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Analysis of histological and immunological parameters of metastatic lymph nodes from colon cancer patients reveals that T-helper 1 type immune response is associated with improved overall survival
    Authors: Joseph Klausner
    Medicine (Baltimore), 2016-11-01;95(45):e5340.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Methylation of Gata3 protein at Arg-261 regulates transactivation of the Il5 gene in T helper 2 cells.
    Authors: Hosokawa H, Kato M, Tohyama H, Tamaki Y, Endo Y, Kimura M, Tumes D, Motohashi S, Matsumoto M, Nakayama K, Tanaka T, Nakayama T
    J Biol Chem, 2015-04-10;290(21):13095-103.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  15. High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human ESC gastruloid cultures
    Authors: KT Minn, YC Fu, S He, S Dietmann, SC George, MA Anastasio, SA Morris, L Solnica-Kr
    Elife, 2020-11-18;9(0):.
  16. Human Early Syncytiotrophoblasts Are Highly Susceptible to SARS-CoV-2 Infection
    Authors: Ruan D, Ye Z, Yuan S et al.
    Cell Reports Medicine

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Human/Mouse GATA-3 Antibody
By Anonymous on 03/20/2023
Application: Immunocytochemistry/Immunofluorescence Sample Tested: BeWo human choriocarcinoma cell line Species: Human

BeWo Cell. Gata3 & Merge with DAPI.