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Human/Mouse Frizzled-1 Antibody

Catalog #: MAB11201
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB11201-050
MAB11201-100
MAB11201-SP
Detection of Frizzled-1 in HEK293 Human Cell Line transfected with Human Frizzled-1 by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Frizzled-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse Frizzled-1 in direct ELISAs and Western blots. Detects human Frizzled-1 in Flow Cytometry. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed. No staining is observed on normal human PBMCs.
Source
Monoclonal Rat IgG2A Clone # 162531
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
Gln72-His248 (Met122Ile)
Accession # O70421
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Frizzled-1 Fc Chimera (Catalog # 1120-FZ)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Frizzled-1 antibody in HEK293 Human Cell Line transfected with Human Frizzled-1 antibody by Flow Cytometry. View Larger

Detection of Frizzled-1 in HEK293 Human Cell Line transfected with Human Frizzled-1 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with Frizzled-1 was stained with Rat Anti-Human/Mouse Frizzled-1 Monoclonal Antibody (Catalog # MAB11201, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Frizzled-1 antibody in HEK293 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Frizzled-1 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled-1 Monoclonal Antibody (Catalog # MAB11201, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-1

The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). Ten mouse and human Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD comprises 642 amino acids and shares 95% identity with the human orthologue. Frizzled-1 mRNA has been detected in relatively large amounts in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung and kidney (3). Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Members of the low density lipoprotein receptor-related protein (LRP) are co-receptors for the Wnt ligands. LRP5/6 serve as co-receptors in the Wnt/Frizzled canonical pathway that alters gene expression via the stabilization of beta -catenin (4, 5). LRP1 may down regulate Wnt-3a/Frizzled-1 signaling in the canonical pathway by sequestering Frizzled-1 (6). Frizzled-1 is one of the purported Wnt-10b receptors whose signaling inhibits adipogenesis in preadipocytes (7). Frizzled-1 may be part of a feedback mechanism to modulate the effects of BMP-2 in mesenchymal cells since upregulation of its expression by BMP-2 counteracts the effects of BMP-2 and Wnt-3a in inducing the expression of the osteoblast differentiation marker, alkaline phosphatase (8).

References
  1. Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59. 
  2. Hsieh, J-C. (2004) Front. Biosci., 9:1333. 
  3. Xu, L. et al. (2001) Matrix Biol. 2:147. 
  4. Tamai, K. et al. (2000) Nature 407:530. 
  5. Pinson, K. et al. (2000) Nature 407:535.
  6. Zielberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
  7. Bennet, C. et al. (2002) J. Biol. Chem. 277:30998.
  8. Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.
Entrez Gene IDs
8321 (Human); 14362 (Mouse)
Alternate Names
DKFZp564G072; FLJ95923; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; Frizzled1; Frizzled-1; fz-1; FZD1; fzE1; hFz1; seven transmembrane spanning receptor; Wnt receptor

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Citations for Human/Mouse Frizzled-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation.
    Authors: Baksh D, Boland GM, Tuan RS
    J. Cell. Biochem., 2007-08-01;101(5):1109-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways
    Authors: Tianzhi Huang, Angel A. Alvarez, Rajendra P. Pangeni, Craig M M. Horbinski, Songjian Lu, Sung-Hak Kim et al.
    Nature Communications

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