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Human/Mouse FGF-16 Antibody

Catalog #: AF1212 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1212
AF1212-SP
FGF‑16 in Human iPS cells.
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Human/Mouse FGF-16 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human FGF-16 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -13, -17, -18, -19, -21, -23, recombinant mouse (rm) FGF-8b, and rmFGF-15 is observed.
Source
Polyclonal Sheep IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FGF‑16 (Catalog # 1212-FG)
Immunohistochemistry
3-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize FGF‑16-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 3-9 µg/mL in the presence of 100 ng/mL Recombinant Human FGF‑16.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry FGF-16 antibody in Human iPS cells by Immunocytochemistry (ICC). View Larger

FGF‑16 in Human iPS cells. FGF-16 was detected in immersion fixed human induced pluripotent stem (iPS) cells differentiated into cardiomyocytes using Sheep Anti-Human/Mouse FGF-16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1212) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry FGF-16 antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

FGF‑16 in Human Heart. FGF-16 was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human/Mouse FGF-16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1212) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry FGF-16 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

FGF‑16 in Mouse Embryo. FGF-16 was detected in perfusion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human/Mouse FGF-16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1212) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to roots of dorsal ganglia and spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization Cell Proliferation Induced by FGF‑16 and Neutralization by Human FGF‑16 Antibody. View Larger

Cell Proliferation Induced by FGF‑16 and Neutralization by Human FGF‑16 Antibody. Recombinant Human FGF-16 (Catalog # 1212-FG) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-16 (100 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human/Mouse FGF-16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1212). The ND50 is typically 3-9 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: FGF-16

Fibroblast growth factor 16 (FGF-16) belongs to the large FGF family which has at least 23 members (1). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (2, 3). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the immunoglobulin (Ig) superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (3).

FGF-16 was originally identified in rat heart tissue by homology based polymerase chain reaction. Human FGF-16 cDNA predicts a 207 aa precursor protein with one N-linked glycosylation site. FGF-16 lacks a typical signal peptide, but is efficiently generated by mechanisms other than the classical protein secretion pathway. Among FGF family members, FGF-16 is most similar to FGF-9, sharing 73% aa sequence homology. Human FGF-16 shares 99% and 98.6% aa sequence identity with the mouse and rat FGF-16, respectively. In rat embryos, FGF-16 message is expressed predominantly in brown adipocytes. In adult animals, it is localized primarily in heart tissue. FGF-16 binds to and activates FGF receptor 4 (4). FGF-16 induces proliferation of primary adipocytes and oligodendrocytes in vitro and stimulates liver weight increase in vivo (4, 5). The expression pattern of FGF-16 and its effect on adipocyte proliferation suggest a role for this protein on the proliferation of embryonic brown adipose tissue (4).

References
  1. Miyake, A. et al. (1998) Biochem. Biophys. Res. Com. 243:148.
  2. Goldfarb, M. (1996) Cytokine and Growth Factor Reviews 7:311.
  3. Green, P. et al. (1996) BioEssays 18:639.
  4. Konishi, M. et al. (2000) J. Biol. Chem. 275:12119.
  5. Danilenko, D.M. et al. (2000) Archiv. Biochem. Biophys. 361:34.
Long Name
Fibroblast Growth Factor 16
Entrez Gene IDs
8823 (Human)
Alternate Names
FGF16; FGF-16; fibroblast growth factor 16

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