Human/Mouse Draxin Antibody Summary
aa 26-349
Accession # Q8NBI3
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Draxin by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Draxin at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Draxin in SH‑SY5Y Human Cell Line. Draxin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Draxin in Mouse Embryo. Draxin was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to diencephalon. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Draxin
Draxin (Dorsal repulsive axon guidance protein; also neucrin) is a secreted, 58 kDa, presumable glycoprotein member of the draxin family of molecules. In mammals, it is expressed in neurons (axons), astroglia, and likely cells of the developing somite. Draxin acts as a Wnt antagonist, apparently by binding to LRP6. The net effect is to block neural crest migration, and the organization of axons into functional tracts or bundles (fasciculation). Mature human Draxin is 324 amino acids (aa) in length. It contains one potential N-linked glycosylation site, followed by a Cys-rich domain (aa 274-333). Mature human Draxin (aa 26-349) shares 79% aa identity with mature mouse Draxin.
Product Datasheets
Citation for Human/Mouse Draxin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons
Authors: Tianxiang Huang, Lena H. Nguyen, Tiffany V. Lin, Xuan Gong, Longbo Zhang, Gi Bum Kim et al.
Neuroscience Research
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