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Human/Mouse Cyclin D1/D2 Antibody

Catalog #: AF4196
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4196
AF4196-SP
Detection of Human/Mouse Cyclin D1/D2 by Western Blot.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Cyclin D1/D2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Cyclin D1/D2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Cyclin D1
Met1-Ile295
Accession # P24385
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse Cyclin D1/D2 antibody by Western Blot. View Larger

Detection of Human/Mouse Cyclin D1/D2 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, U2OS human osteosarcoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse Cyclin D1/D2 Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human Cyclin D1, D2, and D3 (5 ng/lane) were included. A specific band for Cyclin D1 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of Cyclin D1/D2 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Cyclin D1/D2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Goat Anti-Human/Mouse Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4196, filled histogram) or isotype control AA175antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldeyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclin D1/D2

The D-type cyclins (cyclins D1, D2, and D3) and their associated kinases, Cdk4 and Cdk6, play an important role in the progression from G0/G1 to S phase in the mammalian cell cycle. Cyclin D complexes containing Cdk4 or Cdk6 phosphorylate the retinoblastoma protein (pRb). pRb phosphorylation leads to the release of E2F transcription factors, which activate the expression of S-phase genes and thereby induce cell cycle progression. Cyclin D1, independent of Cdk4 activity, functions as a transcriptional modulator by regulating the activity of several transcription factors, such as estrogen receptor, Myb, and STAT3. Cyclin D1 has also been linked to the development and progression of several cancers including breast, bladder, esophagus, and lung. Overexpression of Cyclin D2 has been reported in gastric carcinoma, ovarian granulose cell tumors, and hematopoietic cell cancers, while in breast cancers, cyclin D2 expression is undetectable in 80% of the tumors.

Entrez Gene IDs
595 (Human); 12443 (Mouse); 58919 (Rat)
Alternate Names
BCL1;CCND1;D11S287E;G1/S-specific cyclin-D1;PRAD1;U21B31; Cyclin D1/D2

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Citations for Human/Mouse Cyclin D1/D2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer
    Authors: Guangye Han, Xinjun Zhang, Pei Liu, Quanfeng Yu, Zeyu Li, Qinnan Yu et al.
    International Journal of Oncology
  2. Overexpression of Alpha-1 Antitrypsin Increases the Proliferation of Mesenchymal Stem Cells by Upregulation of Cyclin D1
    Authors: Wolf, B;Muralidharan, P;Lee, MY;Hua, W;Green, E;Wang, H;Strange, C;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Western Blot, ICC
  3. Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers
    Authors: Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J;
    Biosensors
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: IHC
  4. LncRNA SNHG6 regulating Hedgehog signaling pathway and affecting the biological function of gallbladder carcinoma cells through targeting miR-26b-5p
    Authors: XF Liu, K Wang, HC Du
    Eur Rev Med Pharmacol Sci, 2020-07-01;24(14):7598-7611.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. MicroRNA-505 is downregulated in human osteosarcoma and regulates cell proliferation, migration and invasion
    Authors: YJ Liu, W Li, F Chang, JN Liu, JX Lin, DX Chen
    Oncol. Rep., 2017-12-11;39(2):491-500.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Chemokine-Like Receptor 1 Is a Novel Wnt Target Gene that Regulates Mesenchymal Stem Cell Differentiation.
    Authors: Muruganandan S, Govindarajan R, McMullen N, Sinal C
    Stem Cells, 2016-11-11;35(3):711-724.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
    Authors: Q Chen, S Qin, Y Liu, M Hong, CN Qian, ET Keller, J Zhang, Y Lu
    Oncotarget, 2016-10-18;7(42):68140-68150.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Extracellular calcium-sensing receptor/PTH knockout mice colons have increased Wnt/beta-catenin signaling, reduced non-canonical Wnt signaling, and increased susceptibility to azoxymethane-induced aberrant crypt foci.
    Authors: MacLeod R
    Lab Invest, 2013-04-01;93(5):520-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis.
    Authors: Muruganandan S, Parlee SD, Rourke JL, Ernst MC, Goralski KB, Sinal CJ
    J. Biol. Chem., 2011-05-14;286(27):23982-95.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. A Novel Nanofiber Hydrogel Loaded with Platelet-Rich Plasma Promotes Wound Healing Through Enhancing the Survival of Fibroblasts
    Authors: Peng Zhang, Long Zhou, Lei Wang, Qirong Dong
    Medical Science Monitor

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