Human/Mouse Bcl-2 Antibody

Catalog # Availability Size / Price Qty
AF810
AF810-SP
Detection of Human/Mouse Bcl‑2 by Western Blot.
4 Images
Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse Bcl-2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Bcl-2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Bcl-2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse Bcl-2 antibody by Western Blot. View Larger

Detection of Human/Mouse Bcl‑2 by Western Blot. Western blot shows lysates of KG-1 human myeloid leukemia cell line and M-NFS-60 mouse myelogenous leukemia lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Bcl-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Bcl-2, at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry Bcl-2 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Bcl-2 in Human Breast Cancer Tissue. Bcl‑2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human/ Mouse Bcl‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunoprecipitation Immunoprecipitation of Mouse Bcl‑2. View Larger

Immunoprecipitation of Mouse Bcl‑2. Bcl‑2 was immunoprecipitated from lysates (3 x 106cells) of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line following incubation with 3 µg Goat Anti-Human/Mouse Bcl‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810) for 1 hour at 4 °C. Bcl‑2-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated Bcl‑2 was detected by Western blot using 1 µg/mL Goat Anti-Human/Mouse Bcl‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810). View our recommended buffer recipes for immunoprecipitation.

Simple Western Detection of Human Bcl-2 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Bcl‑2 by Simple WesternTM. Simple Western lane view shows lysates of KG-1 human acute myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl-2 at approximately 33 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse Bcl-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-2

Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

 

References
  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Kroemer, G. (1997) Nature Med. 3:614.
Long Name
B Cell Lymphoma/Leukemia 2
Entrez Gene IDs
596 (Human); 12043 (Mouse); 24224 (Rat)
Alternate Names
apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl2; Bcl-2

Product Datasheets

You must select a language.

x

Citations for Human/Mouse Bcl-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Interleukin‑22 regulates gastric cancer cell proliferation through regulation of the JNK signaling pathway
    Authors: Hao Dong, Fengming Zhu, Shilu Jin, Jing Tian
    Experimental and Therapeutic Medicine
  2. Targeting Cell Death Mechanism Specifically in Triple Negative Breast Cancer Cell Lines
    Authors: Lavinia-Lorena Pruteanu, Cornelia Braicu, Dezső Módos, Maria-Ancuţa Jurj, Lajos-Zsolt Raduly, Oana Zănoagă et al.
    International Journal of Molecular Sciences
  3. Targeting of H19/cell adhesion molecules circuitry by GSK-J4 epidrug inhibits metastatic progression in prostate cancer
    Authors: Pecci, V;Troisi, F;Aiello, A;De Martino, S;Carlino, A;Fiorentino, V;Ripoli, C;Rotili, D;Pierconti, F;Martini, M;Porru, M;Pinto, F;Mai, A;Bassi, PF;Grassi, C;Gaetano, C;Pontecorvi, A;Strigari, L;Farsetti, A;Nanni, S;
    Cancer cell international
  4. Knockdown of lncRNA CCAT1 Inhibits the Progression of Colorectal Cancer via hsa-miR-4679 Mediating the Downregulation of GNG10
    Authors: N Wang, J Li, J He, YG Jing, WD Zhao, WJ Yu, J Wang
    Journal of Immunology Research, 2021-12-30;2021(0):8930813.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Lipid storage droplet protein 5 reduces sodium palmitate?induced lipotoxicity in human normal liver cells by regulating lipid metabolism?related factors
    Authors: X Ma, F Cheng, K Yuan, K Jiang, T Zhu
    Mol Med Rep, 2019-06-06;20(2):879-886.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma
    Authors: J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li
    Oncol Lett, 2018-04-13;15(6):9009-9016.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  7. TCDD promotes lung tumors via attenuation of apoptosis through activation of the Akt and ERK1/2 signaling pathways.
    Authors: Chen R, Siao S, Hsu C, Chang C, Chang L, Wu C, Lin P, Wang Y
    PLoS ONE, 2014-06-13;9(6):e99586.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  8. Molecular profiling of cervical cancer progression.
    Authors: Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO
    Br. J. Cancer, 2007-01-29;96(2):321-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
    Authors: Sade H, Krishna S, Sarin A
    J. Biol. Chem., 2003-10-28;279(4):2937-44.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. TGF‑ beta 1 mediates the effects of aspirin on colonic tumor cell proliferation and apoptosis
    Authors: Yuyi Wang, Chi Du, Nan Zhang, Mei Li, Yanyang Liu, Maoyuan Zhao et al.
    Oncology Letters
  11. Securidaca–saponins are natural inhibitors of AKT, MCL-1, and BCL2L1 in cervical cancer cells
    Authors: Titus Chukwuemeka Obasi, Cornelia Braicu, Bogdan Cezar Iacob, Ede Bodoki, Ancuta Jurj, Lajos Raduly et al.
    Cancer Management and Research
  12. Genipin protects against H2O2-induced oxidative damage in�retinal pigment epithelial cells by promoting Nrf2 signaling
    Authors: Hailan Zhao, Ruiqing Wang, Mingxia Ye, Lan Zhang
    International Journal of Molecular Medicine
  13. Influence mechanism of miRNA‑144 on proliferation and apoptosis of osteosarcoma cells
    Authors: Xu Zhang, Zhengwei Li, Wei Ji, Xilong Chen, Qiang Gao, Dajin Li et al.
    Oncology Letters
  14. Protein Synthesis Inhibition Activity of Mesothelin Targeting Immunotoxin LMB-100 Decreases Concentrations of Oncogenic Signaling Molecules and Secreted Growth Factors
    Authors: Salma El-Behaedi, Rebekah Landsman, Michael Rudloff, Emily Kolyvas, Rakan Albalawy, Xianyu Zhang et al.
    Toxins (Basel)

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse Bcl-2 Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse Bcl-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse Bcl-2 Antibody
By Anonymous on 06/17/2024
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Brain (glial cell in hippocampus) Species: Human and Mouse

The conjugation of R&D Systems' Bcl-2 antibody for immunofluorescence has been pivotal in advancing our research on apoptosis regulation. Bcl-2 protein's role in cell survival necessitates accurate detection, making the choice of antibody and conjugation method critical.

We selected R&D Systems' antibody for its established reliability and effectiveness in previous studies. Conjugating the antibody to fluorescent dyes enabled precise localization and visualization of Bcl-2 expression in various cellular contexts. The conjugation process was straightforward, and the resulting fluorescence signals were robust and specific, providing clear insights into protein distribution and dynamics within cells.

Validation involved using appropriate controls and optimizing staining protocols to maximize signal-to-noise ratios. The antibody consistently demonstrated high specificity, effectively distinguishing Bcl-2 from non-specific background fluorescence.

In conclusion, R&D Systems' Bcl-2 antibody conjugated for immunofluorescence has been instrumental in our research, offering reliable and detailed visualization of apoptotic mechanisms. Its performance in conjugation and subsequent imaging has enhanced our understanding of Bcl-2's cellular functions, making it a valuable tool for both basic research and potential therapeutic applications


Human/Mouse Bcl-2 Antibody
By Jothi Jayavel on 01/11/2018
Application: WB Sample Tested: MDA-MBA-231 human breast cancer cell line Species: Human