Human/Mouse ATF2 Antibody

Catalog #: AF4455 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4455
AF4455-SP

Detection of Human/Mouse ATF2 by Western Blot.

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Human/Mouse ATF2 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse ATF2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ATF2
Val270-Ser505
Accession # P15336

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse ATF2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse ATF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ATF2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATF2

ATF2 is a member of the bzip family of transcription factors. ATF2 responds to cAMP activation in cells by either homo or hetero dimerizing with c-Jun and binding to cAMP response elements (TGACGTCA). ATF2 also contains histone acetylase activity that can be induced by ionizing radiation. ATF2 can be phosphorylated by a variety of kinases (JNK, p38, ATM), and this phosphorylation correlates with increased activity.

Long Name

Activating Transcription Factor 2

Entrez Gene IDs

1386 (Human); 11909 (Mouse); 81647 (Rat)

Alternate Names

activating transcription factor 2formerly; ATF2; cAMP responsive element binding protein 2; cAMP-responsive element-binding protein 2; CREB2; CREBP1; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; HB16; MGC111558; mXBP; TREB7